Three-dimensional structure and mimetic-membrane association of consensus 11-amino-acid motif from soybean lea3 protein
文献类型：期刊论文
| 作者 | Xue R ; Liu Y ; Zheng YZ ; Wu YJ ; Li XJ ; Pei FK ; Ni JZ |
| 刊名 | biopolymers
 |
| 出版日期 | 2012 |
| 卷号 | 98期号:1页码:59-66 |
| 关键词 | EMBRYOGENESIS-ABUNDANT PROTEINS TRANSGENIC RICE WATER-DEFICIT NMR DESICCATION GENE TOLERANCE STRESS HVA1 MOLECULES |
| ISSN号 | 0006-3525 |
| 通讯作者 | pei fk |
| 中文摘要 | the occurrence of a highly conserved 11-mer repeating motif in the primary sequence is a major characteristic of group 3 late embryogenesis abundant (lea3) proteins, which are strongly associated with abiotic stress tolerance of the plants. in this study, the three-dimensional structure, mimetic membrane association, and salt effect for consensus 11-mer motif from soybean pm2 protein (lea3) were investigated in sodium dodecyl sulfate (sds) micelles by nmr techniques. it was shown that the 11-mer motif was disordered in aqueous solution, but adopted an alpha-helix in sds micelles. nmr diffusion measurements demonstrated that the 11-mer motif was associated with sds micelles. paramagnetic quenching nmr experiments further revealed the orientation of the 11-mer motif with respect to the mimetic membrane: the ordered n-terminal segment was inserted into the mimetic membrane, and the disordered c-terminal segment was exposed to water. in addition, salt addition could not change the secondary structure of the 11-mer motif, but might slightly alter the relative spatial position of some n-terminal residue atoms. these results implied that the 11-mer motif would take an important role in structural plasticity and membrane stabilization for lea3 proteins. (c) 2011 wiley periodicals, inc. biopolymers (pept sci) 98: 5966, 2012. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000311339000007 |
| 公开日期 | 2013-06-09 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/48421]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Xue R,Liu Y,Zheng YZ,et al. Three-dimensional structure and mimetic-membrane association of consensus 11-amino-acid motif from soybean lea3 protein[J]. biopolymers,2012,98(1):59-66. |
| APA | Xue R.,Liu Y.,Zheng YZ.,Wu YJ.,Li XJ.,...&Ni JZ.(2012).Three-dimensional structure and mimetic-membrane association of consensus 11-amino-acid motif from soybean lea3 protein.biopolymers,98(1),59-66. |
| MLA | Xue R,et al."Three-dimensional structure and mimetic-membrane association of consensus 11-amino-acid motif from soybean lea3 protein".biopolymers 98.1(2012):59-66. |
入库方式： OAI收割
来源：长春应用化学研究所
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