A plant virus substrate induces early upregulation of BMP2 for rapid bone formation
文献类型：期刊论文
| 作者 | Sitasuwan P ; Lee LA ; Bo P ; Davis EN ; Lin Y ; Wang Q |
| 刊名 | integrative biology
 |
| 出版日期 | 2012 |
| 卷号 | 4期号:6页码:651-660 |
| 关键词 | MESENCHYMAL STEM-CELLS TOBACCO-MOSAIC-VIRUS TITANIUM SURFACE-ROUGHNESS OSTEOBLAST-LIKE CELLS MARROW STROMAL CELLS MORPHOGENETIC PROTEIN-2 EXTRACELLULAR-MATRIX IN-VITRO SIGNAL-TRANSDUCTION DIFFERENTIATION |
| ISSN号 | 1757-9694 |
| 通讯作者 | sitasuwan p |
| 中文摘要 | many nanoscale materials have been developed to investigate the effects on stem cell differentiations via topographical and chemical cues for applications in tissue engineering and regenerative medicine. the use of plant viruses as cell supporting substrates has been of particular interest due to the rapid induction of bone marrow derived mesenchymal stem cells (bmscs) towards osteogenic cells. in this study, the role of tobacco mosaic virus (tmv) and its early effects on osteoinduction with particular emphasis on the regulation of bone morphogenetic protein-2 (bmp2) was examined. we observed that the cells on the virus substrate immediately aggregated and formed bone-like nodules within 24 hours. an immediate increase in bmp2 gene and protein expression for cells on the tmv substrate was observed within 8 hours of osteoinduction. moreover, bmp2 expression was highly localized to cells within the cell aggregates. this enhanced differentiation only occurred when tmv was coated on a solid support but not upon adding the virus to the media solution. taken together, the results from this study highlight the potential of virus-based nanomaterials to promote endogenous bmp2 production which may prove to be a unique approach to studying the regulatory mechanisms involved in early osteoblastic differentiation. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000304487300008 |
| 公开日期 | 2013-06-30 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/48546]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Sitasuwan P,Lee LA,Bo P,et al. A plant virus substrate induces early upregulation of BMP2 for rapid bone formation[J]. integrative biology,2012,4(6):651-660. |
| APA | Sitasuwan P,Lee LA,Bo P,Davis EN,Lin Y,&Wang Q.(2012).A plant virus substrate induces early upregulation of BMP2 for rapid bone formation.integrative biology,4(6),651-660. |
| MLA | Sitasuwan P,et al."A plant virus substrate induces early upregulation of BMP2 for rapid bone formation".integrative biology 4.6(2012):651-660. |
入库方式： OAI收割
来源：长春应用化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。