A Novel Single Fluorophore-Labeled Double-Stranded Oligonucleotide Probe for Fluorescence-Enhanced Nucleic Acid Detection Based on the Inherent Quenching Ability of Deoxyguanosine Bases and Competitive Strand-Displacement Reaction
文献类型：期刊论文
| 作者 | Zhang YW ; Tian JQ ; Li HL ; Wang L ; Sun XP |
| 刊名 | journal of fluorescence
 |
| 出版日期 | 2012 |
| 卷号 | 22期号:1页码:43-46 |
| 关键词 | EFFECTIVE SENSING PLATFORM MOLECULAR BEACONS ELECTRON-TRANSFER CARBON NANOTUBES DNA ANALYSIS HYBRIDIZATION BIOMOLECULES |
| ISSN号 | 1053-0509 |
| 通讯作者 | sun xp |
| 中文摘要 | we develop a novel single fluorophore-labeled double-stranded oligonucleotide (ond) probe for rapid, nanostructure-free, fluorescence-enhanced nucleic acid detection for the first time. we further demonstrate such probe is able to well discriminate single-base mutation in nucleic acid. the design takes advantage of an inherent quenching ability of guanine bases. the short strand of the probe is designed with an end-labeled fluorophore that is placed adjacent to two guanines as the quencher located on the long opposite strand, resulting in great quenching of dye fluorescence. in the presence of a target complementary to the long strand of the probe, a competitive strand-displacement reaction occurs and the long strand forms a more stable duplex with the target, resulting in the two strands of the probe being separated from each other. as a consequence of this displacement, the fluorophore and the quencher are no longer in close proximity and dye fluorescence increases, signaling the presence of target. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000302463100005 |
| 公开日期 | 2013-06-30 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/48587]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zhang YW,Tian JQ,Li HL,et al. A Novel Single Fluorophore-Labeled Double-Stranded Oligonucleotide Probe for Fluorescence-Enhanced Nucleic Acid Detection Based on the Inherent Quenching Ability of Deoxyguanosine Bases and Competitive Strand-Displacement Reaction[J]. journal of fluorescence,2012,22(1):43-46. |
| APA | Zhang YW,Tian JQ,Li HL,Wang L,&Sun XP.(2012).A Novel Single Fluorophore-Labeled Double-Stranded Oligonucleotide Probe for Fluorescence-Enhanced Nucleic Acid Detection Based on the Inherent Quenching Ability of Deoxyguanosine Bases and Competitive Strand-Displacement Reaction.journal of fluorescence,22(1),43-46. |
| MLA | Zhang YW,et al."A Novel Single Fluorophore-Labeled Double-Stranded Oligonucleotide Probe for Fluorescence-Enhanced Nucleic Acid Detection Based on the Inherent Quenching Ability of Deoxyguanosine Bases and Competitive Strand-Displacement Reaction".journal of fluorescence 22.1(2012):43-46. |
入库方式： OAI收割
来源：长春应用化学研究所
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