SCREENING FOR alpha-GLUCOSIDASE INHIBITORS FROM COPTIDIS-RIEHMANNIAE HERB COUPLE BY USING ULTRAFILTRATION LIQUID CHROMATOGRAPHY/MASS SPECTROMETRY
文献类型：期刊论文
| 作者 | Zhou H ; Li HL ; Zheng Z ; Song FR ; Xing JP ; Liu ZQ ; Liu SY |
| 刊名 | journal of liquid chromatography & related technologies
 |
| 出版日期 | 2012 |
| 卷号 | 35期号:1-4页码:1-14 |
| 关键词 | TANDEM MASS-SPECTROMETRY RAT PLASMA STEM BARK BERBERINE PALMATINE JATRORRHIZINE ALKALOIDS ALLOXAN RHIZOMA KINASE |
| ISSN号 | 1082-6076 |
| 通讯作者 | liu zq |
| 中文摘要 | alpha-clucosidase inhibitors have been used for the management of type 2 diabetes mellitus (t2dm) for a long lime. the natural alpha-clucosidaseglucosidase inhibitors from chinese herbal medicine have become an attractive therapeutic approach for treating t2dm due to their low toxicity. the assays for the discovery of compounds in natural product extracts, however, mostly needed labor-intensive and time-consuming purification. in the current study, the screening of alpha-glucosidase inhibitors from coptidis-rehmanniae herb couple which was composed of rhizoma coptidis and radix rehmanniae, was investigated by ultrafiltration liquid chromatography! mass spectrometry (uf-lc/ms). as a result, four compounds binding to alpha-glucosidase were observed and identified to be jatrorrhizine, coptisine, palmatine, and berberine. subsequently, the sustained off-resonance irradiation collision-induced dissociation fourier transform mass spectrometry was performed for validation. in addition, the specific binding of ligands to alpha-glucosidase was demonstrated using uf-lc/ms and the ligands were ranked in order of affinity for alpha-glucosidase. the relative affinity order for the ligands was coptisine>jatrorrhizine>palmatine approximate to berberine, which indicated that coptisine and jatrorrhizine exhibited more potent alpha-glucosidase inhibitory activity compared to palmatine and berberine. therefore, uf-lc/ms might provide not only a powerful tool for screening alpha-glucosidase inhibitors in complex samples but also a useful platform for bioactive compounds discovery of the efficient anti-diabetic drugs. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000300967800001 |
| 公开日期 | 2013-06-30 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/48628]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zhou H,Li HL,Zheng Z,et al. SCREENING FOR alpha-GLUCOSIDASE INHIBITORS FROM COPTIDIS-RIEHMANNIAE HERB COUPLE BY USING ULTRAFILTRATION LIQUID CHROMATOGRAPHY/MASS SPECTROMETRY[J]. journal of liquid chromatography & related technologies,2012,35(1-4):1-14. |
| APA | Zhou H.,Li HL.,Zheng Z.,Song FR.,Xing JP.,...&Liu SY.(2012).SCREENING FOR alpha-GLUCOSIDASE INHIBITORS FROM COPTIDIS-RIEHMANNIAE HERB COUPLE BY USING ULTRAFILTRATION LIQUID CHROMATOGRAPHY/MASS SPECTROMETRY.journal of liquid chromatography & related technologies,35(1-4),1-14. |
| MLA | Zhou H,et al."SCREENING FOR alpha-GLUCOSIDASE INHIBITORS FROM COPTIDIS-RIEHMANNIAE HERB COUPLE BY USING ULTRAFILTRATION LIQUID CHROMATOGRAPHY/MASS SPECTROMETRY".journal of liquid chromatography & related technologies 35.1-4(2012):1-14. |
入库方式： OAI收割
来源：长春应用化学研究所
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