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Amplified detection of nucleic acid by G-quadruplex based hybridization chain reaction
文献类型:期刊论文
作者 | Dong, Juan ; Cui, Xin ; Deng, Yun ; Tang, Zhuo |
刊名 | BIOSENSORS & BIOELECTRONICS |
出版日期 | 2012 |
卷号 | 38期号:1页码:258-263 |
ISSN号 | 0956-5663 |
关键词 | Hybridization chain reaction G-quadruplex GQ-HCR Visual chip PCR plus GQ-HCR |
通讯作者 | Deng, Y (reprint author), Chengdu Univ TCM, State Key Lab Breeding Base Systemat Reseach Dev, Chengdu 611137, Peoples R China ; Tang, Zhuo |
产权排序 | 1 |
英文摘要 | A protein-free, isothermal, self-amplified nucleic acid sensing system which was a G-quadruplex integrated hybridization chain reaction (GQ-HCR) system was developed. The G-quadruplex was closed two-thirds in the loop and one-third in the stem of one of the GQ-HCR hairpin probes. In the absence of the target molecule, the GQ-HCR probes stayed as inactive meta-stable hairpin structures and the G-quadruplex was inert. Reversely, the GQ-HCR probes could be cross-opened to start a hybridization chain reaction and the closed G-quadruplex could be released to be free when the GQ-HCR probes came across the target molecule. The GQ-HCR nucleic acid sensing system could detect as low as 7.5 nM ssDNA or RNA by the colorimetric method and 4 nM ssDNA by the fluorometric method. Less than 10 copies of dsDNA template could also be detected when PCR was combined with the GQ-HCR system (PCR+GQ-HCR). Because of these advantages, the GQ-HCR system was also studied for application in visual chip detection to obtain a satisfactory repeatable and specific result. (c) 2012 Elsevier B.V. All rights reserved. |
学科主题 | Biophysics; Biotechnology & Applied Microbiology; Chemistry; Electrochemistry; Science & Technology - Other Topics |
资助信息 | CAS (Hundreds of Talents Program); NSFC [21172215, 21102140]; Innovation Program of the CAS [KSCX2-EW-J-22]; Open Research Fund of State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resource |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000308455700041 |
公开日期 | 2013-07-03 |
源URL | [http://210.75.237.14/handle/351003/23880] |
专题 | 成都生物研究所_天然产物研究 |
推荐引用方式 GB/T 7714 | Dong, Juan,Cui, Xin,Deng, Yun,et al. Amplified detection of nucleic acid by G-quadruplex based hybridization chain reaction[J]. BIOSENSORS & BIOELECTRONICS,2012,38(1):258-263. |
APA | Dong, Juan,Cui, Xin,Deng, Yun,&Tang, Zhuo.(2012).Amplified detection of nucleic acid by G-quadruplex based hybridization chain reaction.BIOSENSORS & BIOELECTRONICS,38(1),258-263. |
MLA | Dong, Juan,et al."Amplified detection of nucleic acid by G-quadruplex based hybridization chain reaction".BIOSENSORS & BIOELECTRONICS 38.1(2012):258-263. |
入库方式: OAI收割
来源:成都生物研究所
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