Identification of flavonoid glycosides in Rosa chinensis flowers by liquid chromatography-tandem mass spectrometry in combination with C-13 nuclear magnetic resonance
文献类型：期刊论文
| 作者 | Qing, Lin-Sen ; Xue, Ying ; Zhang, Jian-Guang ; Zhang, Zhi-Feng ; Liang, Jian ; Jiang, Yan ; Liu, Yi-Ming ; Liao, Xun |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2012 |
| 卷号 | 1249页码:130-137 |
| 关键词 | Rosa chinensis Flavonoid glycoside HPLC-MS/MS C-13 NMR |
| ISSN号 | 0021-9673 |
| 产权排序 | 1 |
| 通讯作者 | Liu, YM (reprint author), Jackson State Univ, Dept Chem & Biochem, 1400 Lynch St, Jackson, MS 39110 USA ; Liao, Xun |
| 英文摘要 | Flowers of Rosa chinensis are widely used in traditional Chinese medicine as well as in food industry. Flavonoid glycosides are believed to be the major components in R. chinensis that are responsible for its antioxidant activities. In this work, a liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed for analysis of flavonoid glycosides presented in ethyl acetate extract of dried R. chinensis flowers. Twelve flavonoid glycosides were separated and detected. By comparing the retention times, UV spectra, and tandem MS fragments with those of respective authentic compounds, eight flavonoid glycosides were unequivocally identified. Although the other four were also identified as flavonoid glycosides, the glycosylation positions could not be determined due to lack of authentic compounds. Fortunately, the glycosylation effects were clearly observed in the C-13 NMR spectrum of the extract. The detailed structural information was, therefore, obtained to identify the four flavonoid glycosides as quercetin-3-O-D-glucoside, quercetin-3-O-D-xyloside, kaempferol-3-O-D-xyloside and quercetin-3-O-D-(6 ''-coumaroyl)-galactoside. These flavonoid glycosides were detected and identified for the first time in this botanic material. This work reports on the first use of C-13 NMR of a mixture to enhance a rapid HPLC-MS/MS analysis. The proposed analytical protocol was validated with a mixture of authentic flavonoid glycosides. (C) 2012 Elsevier B.V. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Chemistry |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [20872137, 21072184]; China Postdoctoral Science Foundation [2011M501312]; US National Institutes of Health [GM089557] |
| 语种 | 英语 |
| WOS记录号 | WOS:000306818700014 |
| 公开日期 | 2013-07-03 |
| 源URL | [http://210.75.237.14/handle/351003/23885]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 推荐引用方式 GB/T 7714 | Qing, Lin-Sen,Xue, Ying,Zhang, Jian-Guang,et al. Identification of flavonoid glycosides in Rosa chinensis flowers by liquid chromatography-tandem mass spectrometry in combination with C-13 nuclear magnetic resonance[J]. JOURNAL OF CHROMATOGRAPHY A,2012,1249:130-137. |
| APA | Qing, Lin-Sen.,Xue, Ying.,Zhang, Jian-Guang.,Zhang, Zhi-Feng.,Liang, Jian.,...&Liao, Xun.(2012).Identification of flavonoid glycosides in Rosa chinensis flowers by liquid chromatography-tandem mass spectrometry in combination with C-13 nuclear magnetic resonance.JOURNAL OF CHROMATOGRAPHY A,1249,130-137. |
| MLA | Qing, Lin-Sen,et al."Identification of flavonoid glycosides in Rosa chinensis flowers by liquid chromatography-tandem mass spectrometry in combination with C-13 nuclear magnetic resonance".JOURNAL OF CHROMATOGRAPHY A 1249(2012):130-137. |
入库方式： OAI收割
来源：成都生物研究所
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