Composition Analysis of Free Fatty Acids from Swertia Species by a Novel Pre-column Fluorescence Labelling Method Using HPLC-FLD
文献类型：期刊论文
| 作者 | Li, Guoliang ; Song, Cuihua ; You, Jinmao ; Zhang, Xiaolong ; Chen, Guang |
| 刊名 | JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
 |
| 出版日期 | 2012 |
| 卷号 | 89期号:4页码:585-595 |
| 关键词 | Free fatty acids HPLC-FLD Pre-column derivatization Swertia species Ultrasonic-assisted extraction |
| ISSN号 | 0003-021X |
| 产权排序 | 3 |
| 通讯作者 | You, JM (reprint author), Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China. |
| 英文摘要 | Free fatty acids (FFA) are basic and indispensable components of medicinal plants. Many researches indicate that the efficacy of medicinal plant requires the presence of FFA. In the present study, ultrasonic-assisted extraction of FFA from Swertia species was optimized by response surface methodology (RSM), ensuring the highest FFA recoveries. A novel pre-column fluorescence labelling method using 2-(5-benzoacridine)ethyl-p-toluenesulfonate (BAETS) as a labelling reagent has been developed for highly sensitive and selective analysis of FFA by HPLC with fluorescent detection (FLD) and online mass spectrometry identification. RSM was also employed to optimize the fluorescence labelling of FFA. HPLC separation of 17 FFA derivatives was carried out on a reversed-phase Hypersil BDS C-8 column (4.6 x 200 mm, 5 mu m, Agilent Co.) with a gradient elution. The detection limits and quantifications were as low as 0.60 and 1.22 ng mL(-1), respectively. FFA analysis from nine Swertia species was performed by the newly developed method, and the FFA composition of these Swertia samples was first reported. |
| 学科主题 | Chemistry; Food Science & Technology |
| 收录类别 | SCI |
| 资助信息 | Chinese Academy of Sciences [328] |
| 语种 | 英语 |
| WOS记录号 | WOS:000305292300005 |
| 公开日期 | 2013-07-03 |
| 源URL | [http://210.75.237.14/handle/351003/23902]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 推荐引用方式 GB/T 7714 | Li, Guoliang,Song, Cuihua,You, Jinmao,et al. Composition Analysis of Free Fatty Acids from Swertia Species by a Novel Pre-column Fluorescence Labelling Method Using HPLC-FLD[J]. JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY,2012,89(4):585-595. |
| APA | Li, Guoliang,Song, Cuihua,You, Jinmao,Zhang, Xiaolong,&Chen, Guang.(2012).Composition Analysis of Free Fatty Acids from Swertia Species by a Novel Pre-column Fluorescence Labelling Method Using HPLC-FLD.JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY,89(4),585-595. |
| MLA | Li, Guoliang,et al."Composition Analysis of Free Fatty Acids from Swertia Species by a Novel Pre-column Fluorescence Labelling Method Using HPLC-FLD".JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY 89.4(2012):585-595. |
入库方式： OAI收割
来源：成都生物研究所
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