Highly selective and sensitive determination of free and total amino acids in Apocynum venetum L. (Luobuma tea) by a developed HPLC-FLD method coupled with pre-column fluorescent labelling
文献类型：期刊论文
| 作者 | Song, Cuihua ; Zhang, Caiqing ; Li, Guoliang ; Zhang, Xiaolong ; Chen, Guang ; You, Jinmao |
| 刊名 | INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION
 |
| 出版日期 | 2012 |
| 卷号 | 63期号:2页码:170-177 |
| 关键词 | amino acids analysis HPLC-FLD analysis pre-column derivatization Apocynum venetum L. |
| ISSN号 | 0963-7486 |
| 产权排序 | 4 |
| 通讯作者 | You, JM (reprint author), Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu, Peoples R China. |
| 英文摘要 | Amino acids (AA) are important chemical constituents of tea leaves remarkably influencing the quality of tea. In this study, free AA and total AA in Apocynum venetum L. (Luobuma tea) were estimated by HPLC equipped with fluorescent detector using 2-[2-(7H-dibenzo[a,g]carbazol-7-yl)-ethoxy] ethyl chloroformate (DBCEEC) as a fluorescent labelling reagent. Different parameters for derivatization and separation were optimized. AA were rapidly derivatized within 3 min at room temperature with DBCEEC. In conjunction with a gradient elution, a baseline resolution of 20 analytes was achieved on a reversed-phase Hypersil BDS C18 column. LC separation for the derivatized AA showed good reproducibility. Twenty AA were detected and showed significant linear responses with correlation coefficients (>0.9992). This developed method offered the low detection limit of 2.88-23.4 fmol. |
| 学科主题 | Food Science & Technology; Nutrition & Dietetics |
| 收录类别 | SCI |
| 资助信息 | Chinese Academy of Sciences [328]; Ministry of Education, Humanities and Social Sciences [09YJA880073] |
| 语种 | 英语 |
| WOS记录号 | WOS:000299745600006 |
| 公开日期 | 2013-07-03 |
| 源URL | [http://210.75.237.14/handle/351003/23911]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 推荐引用方式 GB/T 7714 | Song, Cuihua,Zhang, Caiqing,Li, Guoliang,et al. Highly selective and sensitive determination of free and total amino acids in Apocynum venetum L. (Luobuma tea) by a developed HPLC-FLD method coupled with pre-column fluorescent labelling[J]. INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION,2012,63(2):170-177. |
| APA | Song, Cuihua,Zhang, Caiqing,Li, Guoliang,Zhang, Xiaolong,Chen, Guang,&You, Jinmao.(2012).Highly selective and sensitive determination of free and total amino acids in Apocynum venetum L. (Luobuma tea) by a developed HPLC-FLD method coupled with pre-column fluorescent labelling.INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION,63(2),170-177. |
| MLA | Song, Cuihua,et al."Highly selective and sensitive determination of free and total amino acids in Apocynum venetum L. (Luobuma tea) by a developed HPLC-FLD method coupled with pre-column fluorescent labelling".INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION 63.2(2012):170-177. |
入库方式： OAI收割
来源：成都生物研究所
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