INTERACTION MODE BETWEEN METHYLENE BLUE-Sm(III) COMPLEX AND HERRING SPERM DNA
文献类型：期刊论文
| 作者 | Hu, Ya-min ; Wang, Xing-ming ; Pan, Hai-zhuan ; Ding, Li-sheng |
| 刊名 | BULLETIN OF THE CHEMICAL SOCIETY OF ETHIOPIA
 |
| 出版日期 | 2012 |
| 卷号 | 26期号:3页码:395-405 |
| 关键词 | Methylene blue-Sm(III) Complex Herring sperm DNA Interaction |
| ISSN号 | 1011-3924 |
| 产权排序 | 2 |
| 通讯作者 | Wang, XM (reprint author), SW Univ Sci & Technol, Dept Chem, Sch Mat Sci & Engn, Mianyang 621010, Peoples R China. |
| 英文摘要 | Spectroscopic and viscosity methods were applied to investigate the interaction between methylene blue (MB)-Sm(III) complex and herring sperm DNA by using acridine orange as a spectral probe in Tris-HCl buffer (pH 7.40). By means of molar ratio method, the binding ratios between MB-Sm(III) and DNA were determined as n(Sm(III):)n(MB) = 1:3, n(MB-Sm(III) complex:)n (DNA) = 8 : 1, and the apparent molar absorption coefficient of epsilon(MB-Sm(III)-DNA) was found to be 7.01x10(5) M-1.cm(-1). The bonding constants at different temperatures K-292K(0) = 7.35x10(5) M-1, K-310K(0) = 1.25x10(5) M-1 were obtained by double reciprocal method. Thermodynamic function computation demonstrates that Delta rSm(circle minus) is the primary driving power of the interaction between MB-Sm(III) and herring sperm DNA. By combination analysis of the Scatchard method, circular dichroism spectroscopy and viscosity method, it suggests that the interaction mode between MB-Sm(III) complex and herring sperm DNA are a mixed binding, which contains partial intercalation and electrostatic interaction. |
| 学科主题 | Chemistry |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [30572254] |
| 语种 | 英语 |
| WOS记录号 | WOS:000314400800008 |
| 公开日期 | 2013-07-03 |
| 源URL | [http://210.75.237.14/handle/351003/23921]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 推荐引用方式 GB/T 7714 | Hu, Ya-min,Wang, Xing-ming,Pan, Hai-zhuan,et al. INTERACTION MODE BETWEEN METHYLENE BLUE-Sm(III) COMPLEX AND HERRING SPERM DNA[J]. BULLETIN OF THE CHEMICAL SOCIETY OF ETHIOPIA,2012,26(3):395-405. |
| APA | Hu, Ya-min,Wang, Xing-ming,Pan, Hai-zhuan,&Ding, Li-sheng.(2012).INTERACTION MODE BETWEEN METHYLENE BLUE-Sm(III) COMPLEX AND HERRING SPERM DNA.BULLETIN OF THE CHEMICAL SOCIETY OF ETHIOPIA,26(3),395-405. |
| MLA | Hu, Ya-min,et al."INTERACTION MODE BETWEEN METHYLENE BLUE-Sm(III) COMPLEX AND HERRING SPERM DNA".BULLETIN OF THE CHEMICAL SOCIETY OF ETHIOPIA 26.3(2012):395-405. |
入库方式： OAI收割
来源：成都生物研究所
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