A practical high-throughput screening system for feruloyl esterases: Substrate design and evaluation
文献类型：期刊论文
| 作者 | Zhang, Shuai-Bing ; Ma, Xiao-Feng ; Pei, Xiao-Qiong ; Liu, Jing-Yuan ; Shao, Hua-Wu ; Wu, Zhong-Liu |
| 刊名 | JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
 |
| 出版日期 | 2012 |
| 卷号 | 74期号:1-2页码:36-40 |
| 关键词 | Feruloyl esterases High-throughput screening Pichia pastoris 2-Chloro-4-nitrophenol Umbelliferyl |
| ISSN号 | 1381-1177 |
| 产权排序 | 1 |
| 通讯作者 | Shao, Hua-Wu ; Wu, ZL (reprint author), Chinese Acad Sci, Chengdu Inst Biol, 9 S Renmin Rd,4th Sect, Chengdu 610041, Sichuan, Peoples R China. |
| 英文摘要 | Feruloyl esterases (FAEs) are a group of important industrial enzymes, which could hydrolyze the ester bonds between hydroxycinnamic acids and arabinose or galactose in hemicellulose present in plant cell walls. To establish a practical high-throughput screening system of feruoyl esterases, two new substrates with modified chromo- or fluoro-phore, 2-chloro-4-nitrophenyl ferulate (CNPF; 1b) and umbelliferyl 5-O-feruolyl-alpha-L-arabinofuranoside (UFA; 2a), were designed and synthesized. Both substrates provided significantly improved signal-to-noise ratio compared with previously known structural analogues. The chromogenic substrate CNPF could be readily adapted to the high-throughput screening of feruloyl esterase A from Aspergillus niger, one of the most studied FAEs, with the coefficient of variance of as low as 10.3% as determined for a single sequence library. (C) 2011 Elsevier B.V. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Chemistry |
| 收录类别 | SCI |
| 资助信息 | Chinese Academy of Sciences [KSCX1-YW-11B2]; National Natural Science Foundation of China [20802073, 21072183]; Province Science Foundation of Sichuan, China [08ZQ026-023, 2010SZ0128] |
| 语种 | 英语 |
| WOS记录号 | WOS:000297609900007 |
| 公开日期 | 2013-07-03 |
| 源URL | [http://210.75.237.14/handle/351003/23938]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 推荐引用方式 GB/T 7714 | Zhang, Shuai-Bing,Ma, Xiao-Feng,Pei, Xiao-Qiong,et al. A practical high-throughput screening system for feruloyl esterases: Substrate design and evaluation[J]. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,2012,74(1-2):36-40. |
| APA | Zhang, Shuai-Bing,Ma, Xiao-Feng,Pei, Xiao-Qiong,Liu, Jing-Yuan,Shao, Hua-Wu,&Wu, Zhong-Liu.(2012).A practical high-throughput screening system for feruloyl esterases: Substrate design and evaluation.JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,74(1-2),36-40. |
| MLA | Zhang, Shuai-Bing,et al."A practical high-throughput screening system for feruloyl esterases: Substrate design and evaluation".JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 74.1-2(2012):36-40. |
入库方式： OAI收割
来源：成都生物研究所
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