Metabolic engineering of tomato for high-yield production of astaxanthin
文献类型：期刊论文
| 作者 | Huang, Jun-Chao1 ; Zhong, Yu-Juan2,3; Liu, Jin2; Sandmann, Gerhard4; Chen, Feng5
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| 刊名 | METABOLIC ENGINEERING
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| 出版日期 | 2013-05-01 |
| 卷号 | 17页码:59-67 |
| 关键词 | Astaxanthin beta-carotene ketolase beta-carotene hydroxylase Tomato |
| ISSN号 | 1096-7176 |
| 通讯作者 | Chen, F (reprint author), Peking Univ, Coll Engn, Inst Food & Bioresource Engn, Beijing 100871, Peoples R China. ; sfchencoe@pku.edu.cn |
| 英文摘要 | Dietary carotenoids have been shown to be beneficial to health by decreasing the risk of many diseases. Attempts to enhance carotenoids in food crops have been successful although higher plants appear to resist big changes of carotenoid biosynthesis by metabolic engineering. Here we report the generation of a more nutritious tomato by modifying the intrinsic carotenes to astaxanthin, a high-value ketocarotenoid rarely found in plants. This was achieved by co-expression of the algal beta-carotene ketolase from Chlamydomonas reinhardtii and beta-carotene hydroxylase from Haematococcus pluvialis, a unique pair of enzymes identified to co-operate perfectly in converting p-carotene to astaxanthin by functional complementation in Escherichia coli. Expression of the two enzymes in tomato up-regulated most intrinsic carotenogenic genes, and efficiently directed carbon flux into carotenoids, leading to massive accumulations of mostly free astaxanthin in leaves (3.12 mg/g) but esterified astaxanthin in fruits (16.1 mg/g) and a 16-fold increase of total carotenoid capacity therein without affecting the plant normal growth and development. This study opened up the possibility of employing crop plants as green factories for economical production of astaxanthin. (C) 2013 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biotechnology & Applied Microbiology |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | BETA-CAROTENE KETOLASE ; HAEMATOCOCCUS-PLUVIALIS ; ESCHERICHIA-COLI ; PLANTS ; BIOSYNTHESIS ; GENE ; EXPRESSION ; PATHWAY ; FRUIT ; CANTHAXANTHIN |
| 收录类别 | SCI |
| 资助信息 | Peking University; Yunnan high talents program |
| 语种 | 英语 |
| WOS记录号 | WOS:000318583700007 |
| 公开日期 | 2013-07-24 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/16605]  |
| 专题 | 昆明植物研究所_资源植物与生物技术所级重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, Kunming, Peoples R China 2.Univ Hong Kong, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China 3.Guangdong Acad Agr Sci, Inst Vegetable Res, Guangzhou, Guangdong, Peoples R China 4.Goethe Univ Frankfurt, Biosynth Grp, D-60054 Frankfurt, Germany 5.Peking Univ, Coll Engn, Inst Food & Bioresource Engn, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Jun-Chao,Zhong, Yu-Juan,Liu, Jin,et al. Metabolic engineering of tomato for high-yield production of astaxanthin[J]. METABOLIC ENGINEERING,2013,17:59-67. |
| APA | Huang, Jun-Chao,Zhong, Yu-Juan,Liu, Jin,Sandmann, Gerhard,&Chen, Feng.(2013).Metabolic engineering of tomato for high-yield production of astaxanthin.METABOLIC ENGINEERING,17,59-67. |
| MLA | Huang, Jun-Chao,et al."Metabolic engineering of tomato for high-yield production of astaxanthin".METABOLIC ENGINEERING 17(2013):59-67. |
入库方式： OAI收割
来源：昆明植物研究所
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