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Chinese Academy of Sciences Institutional Repositories Grid
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  2. 烟台海岸带研究所
  3. 中国科学院烟台海岸带研究所
  4. 中科院海岸带环境过程与生态修复重点实验室
Quick identification and quantification of Proteus mirabilis by polymerase chain reaction (PCR) assays

文献类型：期刊论文

作者Zhang, Weiwei1,2; Niu, Zongliang1,2; Yin, Kun1,2,3; Liu, Ping1,2; Chen, Lingxin1,2,4
刊名ANNALS OF MICROBIOLOGY
出版日期2013-06-01
卷号63期号:2页码:683-689
关键词Proteus mirabilis ureR Polymerase chain reaction (PCR) Real-time PCR
ISSN号1590-4261
产权排序[Zhang, Weiwei; Niu, Zongliang; Yin, Kun; Liu, Ping; Chen, Lingxin] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China; [Zhang, Weiwei; Niu, Zongliang; Yin, Kun; Liu, Ping; Chen, Lingxin] Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China; [Yin, Kun] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China
通讯作者Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Yantai 264003, Shandong, Peoples R China. lxchen@yic.ac.cn
中文摘要Proteus mirabilis is an opportunistic pathogen that can cause urinary tract infection in human beings. The accurate and rapid identification and quantification of P. mirabilis is necessary for early treatment. In this study, a pair of specific primers according to the conserved ureR sequence of P. mirabilis was designed and novel systems which consisted of a polymerase chain reaction (PCR) and a real-time PCR to identify and quantify P. mirabilis were developed. For the qualitative identification by ordinary PCR, a 225-bp DNA product was amplified from P. mirabilis and separated on an agarose gel. The corresponding DNA product is present in three P. mirabilis strains isolated from different geographical locations, but is absent in 20 strains representing 18 different species, including the ureR homolog contained Providencia stuartii and Escherichia coli strains, the other common pathogens Klebsiella sp., Edwarsiella sp., Vibrio sp., Enterobacter sp., and Escherichia sp., and other environmental bacteria Pseudomonas sp. and Acinetobacter sp. Proteus mirabilis at concentrations higher than 1.0 x 10(3) CFU ml(-1) was detectable by ordinary PCR; P. mirabilis at concentrations higher than 10 CFU ml(-1) was quantified by real-time PCR. The specific, sensitive and time-efficient PCR methods were demonstrated to be applicable to rapid identification and quantification of P. mirabilis.
英文摘要Proteus mirabilis is an opportunistic pathogen that can cause urinary tract infection in human beings. The accurate and rapid identification and quantification of P. mirabilis is necessary for early treatment. In this study, a pair of specific primers according to the conserved ureR sequence of P. mirabilis was designed and novel systems which consisted of a polymerase chain reaction (PCR) and a real-time PCR to identify and quantify P. mirabilis were developed. For the qualitative identification by ordinary PCR, a 225-bp DNA product was amplified from P. mirabilis and separated on an agarose gel. The corresponding DNA product is present in three P. mirabilis strains isolated from different geographical locations, but is absent in 20 strains representing 18 different species, including the ureR homolog contained Providencia stuartii and Escherichia coli strains, the other common pathogens Klebsiella sp., Edwarsiella sp., Vibrio sp., Enterobacter sp., and Escherichia sp., and other environmental bacteria Pseudomonas sp. and Acinetobacter sp. Proteus mirabilis at concentrations higher than 1.0 x 10(3) CFU ml(-1) was detectable by ordinary PCR; P. mirabilis at concentrations higher than 10 CFU ml(-1) was quantified by real-time PCR. The specific, sensitive and time-efficient PCR methods were demonstrated to be applicable to rapid identification and quantification of P. mirabilis.
学科主题Biotechnology & Applied Microbiology; Microbiology
研究领域[WOS]Biotechnology & Applied Microbiology ; Microbiology
关键词[WOS]REAL-TIME PCR ; SYBR GREEN-I ; ANTIBIOTIC SUSCEPTIBILITY ; RAPID DETECTION ; VIBRIO-HARVEYI ; RIBOSOMAL-RNA ; URINARY-TRACT ; ENTEROBACTERIACEAE ; SAMPLES ; SPECIMENS
收录类别SCI
资助信息Chinese Academy of Sciences [KZCX2-EW-206]; National Natural Science Foundation of China (NSFC) [20975089]; Department of Science and Technology of Yantai City of China [2010235]; Doctoral Foundation of Shandong Province [BS2011SW056]; 100 Talents Program of the Chinese Academy of Sciences
原文出处10.1007/s13213-012-0520-x
语种英语
WOS记录号WOS:000319074600032
公开日期2013-08-15
源URL[http://ir.yic.ac.cn/handle/133337/6659]  
专题烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China
2.Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Weiwei,Niu, Zongliang,Yin, Kun,et al. Quick identification and quantification of Proteus mirabilis by polymerase chain reaction (PCR) assays[J]. ANNALS OF MICROBIOLOGY,2013,63(2):683-689.
APA Zhang, Weiwei,Niu, Zongliang,Yin, Kun,Liu, Ping,&Chen, Lingxin.(2013).Quick identification and quantification of Proteus mirabilis by polymerase chain reaction (PCR) assays.ANNALS OF MICROBIOLOGY,63(2),683-689.
MLA Zhang, Weiwei,et al."Quick identification and quantification of Proteus mirabilis by polymerase chain reaction (PCR) assays".ANNALS OF MICROBIOLOGY 63.2(2013):683-689.

入库方式： OAI收割

来源：烟台海岸带研究所

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