Rapid and label-free detection of porcine reproductive and respiratory syndrome virus on nanoscale by biosensor based on imaging ellipsometry
文献类型：期刊论文
| 作者 | Huang CH; Hou CJ; Huo DQ; Chen YY ; Jin G(靳刚)
|
| 刊名 | INTEGRATED FERROELECTRICS
 |
| 出版日期 | 2013 |
| 卷号 | 145期号:1页码:122-129 |
| 通讯作者邮箱 | huodq@cqu.edu.cn; gajin@imech.ac.cn |
| 关键词 | Biosensor Imaging Ellipsometry PRRSV |
| ISSN号 | 1058-4587 |
| 产权排序 | [Huang, Cheng-Hong; Hou, Changjun; Huo, Danqun] Chongqing Univ, Bioengn Coll, Minist Educ, Key Lab Biorheol Sci & Technol, Chongqing 400030, Peoples R China; [Chen, Yanyan] Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou 215123, Peoples R China; [Jin, Gang] Chinese Acad Sci, Inst Mech, Beijing 100190, Peoples R China |
| 通讯作者 | Huo, DQ (reprint author), Chongqing Univ, Bioengn Coll, Minist Educ, Key Lab Biorheol Sci & Technol, Chongqing 400030, Peoples R China. |
| 合作状况 | 国内 |
| 中文摘要 | This study describes a simple and rapid method for virus detection on nanoscale measurement utilizing biosensor based on imaging ellipsometry. Porcine reproductive and respiratory syndrome virus (PRRSV) was served as an experimental model for demonstration. Staphylococcal protein A was firstly attached onto carboxyl-modified silicon substrate and purified anti-PRRSV IgG obtained from hyperimmune sera combined with Protein A for oriented immobilization to form capture layer. PRRSV in samples can then be specifically grabbed onto the capture layer. The signal (grayscale value) after virus combination is intuitively quantified by imaging ellipsometry. The minimum detection limit was as low as 2.4 x 10(3) median cell infectious dose (CCID50 mL(-1)) infectious virus. Application the proposed method to 20 clinic samples demonstrated that the result was significant correlated (R = 0.85) with virus isolation. The detection time was only 20min expended. |
| 学科主题 | 交叉与边缘领域的力学 |
| 分类号 | Q4 |
| 收录类别 | SCI ; EI |
| 资助信息 | laboratory of Sichuan, China [NJ2009-04] |
| 原文出处 | http://dx.doi.org/10.1080/10584587.2013.788963 |
| 语种 | 英语 |
| WOS记录号 | WOS:000321687200016 |
| 公开日期 | 2013-08-21 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/47361]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Huang CH,Hou CJ,Huo DQ,et al. Rapid and label-free detection of porcine reproductive and respiratory syndrome virus on nanoscale by biosensor based on imaging ellipsometry[J]. INTEGRATED FERROELECTRICS,2013,145(1):122-129. |
| APA | Huang CH,Hou CJ,Huo DQ,Chen YY,&Jin G.(2013).Rapid and label-free detection of porcine reproductive and respiratory syndrome virus on nanoscale by biosensor based on imaging ellipsometry.INTEGRATED FERROELECTRICS,145(1),122-129. |
| MLA | Huang CH,et al."Rapid and label-free detection of porcine reproductive and respiratory syndrome virus on nanoscale by biosensor based on imaging ellipsometry".INTEGRATED FERROELECTRICS 145.1(2013):122-129. |
入库方式： OAI收割
来源：力学研究所
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