Two dimensional barcode-inspired automatic analysis for arrayed microfluidic immunoassays
文献类型：期刊论文
| 作者 | Zhang Y; Qiao LB; Ren YK; Wang XW(王绪伟) ; Gao M; Tang YF; Xi JJ; Fu TM; Jiang XY
|
| 刊名 | BIOMICROFLUIDICS
 |
| 出版日期 | 2013-05-09 |
| 卷号 | 7期号:3页码:034110/1-034110/12 |
| 通讯作者邮箱 | tmfu@pku.edu.cn; xingyujiang@nanoctr.cn |
| ISSN号 | 1932-1058 |
| 关键词 | Bar codes Two dimensional Antigen/antibody interactions Automatic analysis Biochemical reactions Lab-on-a-chip devices Microfluidic array Microfluidic immunoassay Quantitative information Universal approach |
| 通讯作者 | Fu, TM (reprint author), Peking Univ, Coll Engn, Beijing 100871, Peoples R China. |
| 产权排序 | [Zhang, Yi; Ren, Yunke; Xi, Jianzhong Jeff; Fu, Tzung-May] Peking Univ, Coll Engn, Beijing 100871, Peoples R China; [Zhang, Yi; Ren, Yunke; Xi, Jianzhong Jeff; Fu, Tzung-May] Peking Univ, Sch Phys, Beijing 100871, Peoples R China; [Zhang, Yi; Tang, Yunfang; Jiang, Xingyu] Natl Ctr Nanosci & Technol, Beijing 100190, Peoples R China; [Qiao, Lingbo; Gao, Ming] Tsinghua Univ, Dept Engn Phys, Beijing 100084, Peoples R China; [Qiao, Lingbo] Tsinghua Univ, Key Lab Particle & Radiat Imaging, Minist Educ, Beijing 100084, Peoples R China; [Wang, Xuwei] Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, Beijing 100080, Peoples R China |
| 合作状况 | 国内 |
| 中文摘要 | The usability of many high-throughput lab-on-a-chip devices in point-of-care applications is currently limited by the manual data acquisition and analysis process, which are labor intensive and time consuming. Based on our original design in the biochemical reactions, we proposed here a universal approach to perform automatic, fast, and robust analysis for high-throughput array-based microfluidic immunoassays. Inspired by two-dimensional (2D) barcodes, we incorporated asymmetric function patterns into a microfluidic array. These function patterns provide quantitative information on the characteristic dimensions of the microfluidic array, as well as mark its orientation and origin of coordinates. We used a computer program to perform automatic analysis for a high-throughput antigen/antibody interaction experiment in 10 s, which was more than 500 times faster than conventional manual processing. Our method is broadly applicable to many other microchannel-based immunoassays. |
| 学科主题 | 交叉与边缘领域的力学 |
| 分类号 | 一类 |
| 资助信息 | National Natural Science Foundation of China [21025520]; Chinese Academy of Sciences [KJCX2-YW-M15]; Ministry of Science and Technology of the People's Republic of China [2012AA030608] |
| 收录类别 | SCI ; EI |
| 原文出处 | http://dx.doi.org/10.1063/1.4811278 |
| 语种 | 英语 |
| WOS记录号 | WOS:000321145800011 |
| 公开日期 | 2013-08-21 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/47358]  |
| 专题 | 力学研究所_非线性力学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Zhang Y,Qiao LB,Ren YK,et al. Two dimensional barcode-inspired automatic analysis for arrayed microfluidic immunoassays[J]. BIOMICROFLUIDICS,2013,7(3):034110/1-034110/12. |
| APA | Zhang Y.,Qiao LB.,Ren YK.,Wang XW.,Gao M.,...&Jiang XY.(2013).Two dimensional barcode-inspired automatic analysis for arrayed microfluidic immunoassays.BIOMICROFLUIDICS,7(3),034110/1-034110/12. |
| MLA | Zhang Y,et al."Two dimensional barcode-inspired automatic analysis for arrayed microfluidic immunoassays".BIOMICROFLUIDICS 7.3(2013):034110/1-034110/12. |
入库方式： OAI收割
来源：力学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。