Measuring Receptor/Ligand Interaction at the Single-Bond Level: Experimental and Interpretative Issues
文献类型：期刊论文
| 作者 | Zhu C(朱承); Long M(龙勉) ; Chesla SE; Bongrand P
|
| 刊名 | Annals of Biomedical Engineering
 |
| 出版日期 | 2002 |
| 卷号 | 30期号:3页码:305-314 |
| ISSN号 | 0090-6964 |
| 通讯作者 | Zhu, C (reprint author), Georgia Inst Technol, George W Woodruff Sch Mech Engn, Atlanta, GA 30332 USA. |
| 英文摘要 | There is increased interest in measuring kinetic rates, lifetimes, and rupture forces of single receptor/ligand bonds. Valuable insights have been obtained from previous experiments attempting such measurements. However, it remains difficult to know with sufficient certainty that single bonds were indeed measured. Using exemplifying data, evidence supporting single-bond observation is examined and caveats in the experimental design and data interpretation are identified. Critical issues preventing definitive proof and disproof of single-bond observation include complex binding schemes, multimeric interactions, clustering, and heterogeneous surfaces. It is concluded that no single criterion is sufficient to ensure that single bonds are actually observed. However, a cumulative body of evidence may provide reasonable confidence. 0 2002 Biomedical Engineering Society. |
| 学科主题 | 力学 |
| 类目[WOS] | Engineering, Biomedical |
| 研究领域[WOS] | Engineering |
| 关键词[WOS] | SELECTIN GLYCOPROTEIN LIGAND-1 ; ATOMIC-FORCE MICROSCOPY ; SHEAR-FLOW ; CELL-ADHESION ; P-SELECTIN ; MOLECULAR ADHESION ; KINETIC RATES ; BINDING ; LIFETIME ; MEMBRANE |
| 收录类别 | SCI ; EI |
| 语种 | 英语 |
| WOS记录号 | WOS:000175849500004 |
| 公开日期 | 2007-06-15 ; 2007-12-05 ; 2009-06-23 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/15995]  |
| 专题 | 力学研究所_力学所知识产出(1956-2008) |
| 推荐引用方式 GB/T 7714 | Zhu C,Long M,Chesla SE,et al. Measuring Receptor/Ligand Interaction at the Single-Bond Level: Experimental and Interpretative Issues[J]. Annals of Biomedical Engineering,2002,30(3):305-314. |
| APA | 朱承,龙勉,Chesla SE,&Bongrand P.(2002).Measuring Receptor/Ligand Interaction at the Single-Bond Level: Experimental and Interpretative Issues.Annals of Biomedical Engineering,30(3),305-314. |
| MLA | 朱承,et al."Measuring Receptor/Ligand Interaction at the Single-Bond Level: Experimental and Interpretative Issues".Annals of Biomedical Engineering 30.3(2002):305-314. |
入库方式： OAI收割
来源：力学研究所
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