KF polymerase-based fluorescence aptasensor for the label-free adenosine detection
文献类型：期刊论文
| 作者 | Liao DL ; Jiao HP ; Wang B ; Lin Q ; Yu C |
| 刊名 | analyst
 |
| 出版日期 | 2012 |
| 卷号 | 137期号:4页码:978-982 |
| 关键词 | IN-VITRO SELECTION BASAL GANGLIA APTAMER DNA SENSORS LIGANDS AMPLIFICATION NANOPARTICLES DIAGNOSTICS MOLECULES |
| ISSN号 | 0003-2654 |
| 通讯作者 | yu c |
| 中文摘要 | we have developed a simple, inexpensive, and label-free method for the selective detection of adenosine. klenow fragment polymerase (kf polymerase) is a commonly-used 5' to 3' dna polymerase, it also has 3' to 5' exonuclease activity that can digest single-stranded dna. an adenosine binding dna aptamer was employed, the aptamer was split into two pieces of single-stranded dna (aptamer-a1 + aptamer-a2). without the addition of adenosine, aptamer-a1 and aptamer-a2 existed as single-stranded dna which could be efficiently degraded by the exonuclease activity of kf polymerase. much reduced background fluorescence was obtained when sybr green dye was added. however, in the presence of adenosine, aptamer-a1 and aptamer-a2 bound to adenosine, and hybridization of the complementary sequences resulted in the formation of a duplex dna structure, which could initiate dna polymerization. the addition of sybr green dye resulted in a very high fluorescence enhancement, which could be used for the quantification of adenosine. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000299476300027 |
| 公开日期 | 2013-08-29 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/48715]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Liao DL,Jiao HP,Wang B,et al. KF polymerase-based fluorescence aptasensor for the label-free adenosine detection[J]. analyst,2012,137(4):978-982. |
| APA | Liao DL,Jiao HP,Wang B,Lin Q,&Yu C.(2012).KF polymerase-based fluorescence aptasensor for the label-free adenosine detection.analyst,137(4),978-982. |
| MLA | Liao DL,et al."KF polymerase-based fluorescence aptasensor for the label-free adenosine detection".analyst 137.4(2012):978-982. |
入库方式： OAI收割
来源：长春应用化学研究所
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