Offline preparative 2-D polar-copolymerized reversed-phase chromatography x zwitterionic hydrophilic interaction chromatography for effective purification of polar compounds from Caulis Polygoni Multiflori
文献类型：期刊论文
| 作者 | Liu, Dian1,2; Jin, Hongli2; Wang, Jixia2; Zhou, Han2; Liu, Yanfang2; Feng, Jiatao2; Liang, Xinmiao2 |
| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2019-06-15 |
| 卷号 | 1118页码:70-77 |
| 关键词 | Caulis Polygoni Multiflori Polar compounds Polar-copolymerized reversed-phase chromatography Zwitterionic hydrophilic interaction chromatography |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2019.04.039 |
| 通讯作者 | Liu, Yanfang(liuyanfang@dicp.ac.cn) ; Liang, Xinmiao(liangxm@dicp.ac.cn) |
| 英文摘要 | Polar compounds are abundant in the water decoction of Traditional Chinese Medicines (TCMs) and possess important biological activities. However, purifying these compounds has faced great difficulties largely due to poor retention and insufficient selectivity. To solve this problem, an offline orthogonal 2-D RPLC coupled with hydrophilic interaction chromatography (HILIC) method was developed to achieve purification of polar compounds from Caulis Polygoni Multiflori. A polar-copolymerized XAqua C18 column and a zwitterionic Click XIon column exhibited satisfactory retention and separation for polar compounds. Therefore, they were adopted to construct the offline 2-D LC system. Furthermore, the method presented a high orthogonality, which was calculated to be 72%. The)(Aqua C18 column was used in the first dimension to fractionate the 5.1 g polar fraction with a recovery of 85% within 10 h. In the second dimension, three representative fractions were purified using the Click XIon column. Finally, three compounds with purity higher than 95% were identified for the first time from this plant. This offline 2-D RPLC/HILIC method was shown to be an effective approach to purifying polar compounds from Caulis Polygoni Multiflori. |
| WOS关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY ; BASIC COMPOUNDS ; MASS-SPECTROMETRY ; C18 COLUMN ; SEPARATION ; ALKALOIDS ; ONLINE ; COMPONENTS ; SYSTEM |
| 资助项目 | State Key Program of National Natural Science of China[U1508221] ; National Science Foundation of China[31670374] ; National Science Foundation of China[81403100] ; Innovation Program of science and research from DICP, CAS[DICP TMSR201601] ; Funding for the Construction of DICP-CMC Innovation Institute of Medicine |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000469153600010 |
| 出版者 | ELSEVIER SCIENCE BV |
| 资助机构 | State Key Program of National Natural Science of China ; State Key Program of National Natural Science of China ; National Science Foundation of China ; National Science Foundation of China ; Innovation Program of science and research from DICP, CAS ; Innovation Program of science and research from DICP, CAS ; Funding for the Construction of DICP-CMC Innovation Institute of Medicine ; Funding for the Construction of DICP-CMC Innovation Institute of Medicine ; State Key Program of National Natural Science of China ; State Key Program of National Natural Science of China ; National Science Foundation of China ; National Science Foundation of China ; Innovation Program of science and research from DICP, CAS ; Innovation Program of science and research from DICP, CAS ; Funding for the Construction of DICP-CMC Innovation Institute of Medicine ; Funding for the Construction of DICP-CMC Innovation Institute of Medicine ; State Key Program of National Natural Science of China ; State Key Program of National Natural Science of China ; National Science Foundation of China ; National Science Foundation of China ; Innovation Program of science and research from DICP, CAS ; Innovation Program of science and research from DICP, CAS ; Funding for the Construction of DICP-CMC Innovation Institute of Medicine ; Funding for the Construction of DICP-CMC Innovation Institute of Medicine ; State Key Program of National Natural Science of China ; State Key Program of National Natural Science of China ; National Science Foundation of China ; National Science Foundation of China ; Innovation Program of science and research from DICP, CAS ; Innovation Program of science and research from DICP, CAS ; Funding for the Construction of DICP-CMC Innovation Institute of Medicine ; Funding for the Construction of DICP-CMC Innovation Institute of Medicine |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/171945]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Liu, Yanfang; Liang, Xinmiao |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Dian,Jin, Hongli,Wang, Jixia,et al. Offline preparative 2-D polar-copolymerized reversed-phase chromatography x zwitterionic hydrophilic interaction chromatography for effective purification of polar compounds from Caulis Polygoni Multiflori[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2019,1118:70-77. |
| APA | Liu, Dian.,Jin, Hongli.,Wang, Jixia.,Zhou, Han.,Liu, Yanfang.,...&Liang, Xinmiao.(2019).Offline preparative 2-D polar-copolymerized reversed-phase chromatography x zwitterionic hydrophilic interaction chromatography for effective purification of polar compounds from Caulis Polygoni Multiflori.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1118,70-77. |
| MLA | Liu, Dian,et al."Offline preparative 2-D polar-copolymerized reversed-phase chromatography x zwitterionic hydrophilic interaction chromatography for effective purification of polar compounds from Caulis Polygoni Multiflori".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1118(2019):70-77. |
入库方式： OAI收割
来源：大连化学物理研究所
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