Culture of yeast cells immobilized by alginate-chitosan microcapsules in aqueous-organic solvent biphasic system
文献类型：期刊论文
| 作者 | Hou Dandan1; Yu Weiting2; Zhang Demeng3; Zhao Lili3; Liu Xiudong1; Ma Xiaojun4 |
| 刊名 | JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 出版日期 | 2019-05-01 |
| 卷号 | 37期号:3页码:863-870 |
| ISSN号 | 2096-5508 |
| 关键词 | alginate-chitosan (AC) microcapsules immobilization biocatalysis aqueous-solvent biphasic system cell growth |
| DOI | 10.1007/s00343-019-8126-9 |
| 通讯作者 | Yu Weiting(yuwt@dicp.ac.cn) ; Liu Xiudong(liuxiudong@dlu.edu.cn) |
| 英文摘要 | Immobilization biocatalysis is a potential technology to improve the activity and stability of biocatalysts in nonaqueous systems for efficient industrial production. Alginate-chitosan (AC) microcapsules were prepared as immobilization carriers by emulsification-internal gelation and complexation reaction, and their contribution on facilitating the growth and metabolism of yeast cells were testified successfully in culture medium-solvent biphasic systems. The cell growth in AC microcapsules is superior to that in alginate beads, and the cells in both immobilization carriers maintain much higher activity than free cells, which demonstrates AC microcapsules can confer yeast cells the ability to resist the adverse effect of solvent. Moreover, the performance of AC microcapsules in biphasic systems could be improved by adjusting the formation of outer polyelectrolyte complex (PEC) membrane to promote the cell growth and metabolic ability under the balance of resisting solvent toxicity and permitting substrate diffusion. Therefore, these findings are quite valuable for applying AC microcapsules as novel immobilization carriers to realize the biotransformation of value-added products in aqueous-solvent biphasic systems. |
| WOS关键词 | BIOCATALYSIS ; MICROENCAPSULATION ; VIABILITY |
| 资助项目 | National Natural Science Foundation of China[21276033] ; Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances[SKL-BASS1707] ; Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances[SKL-BASS1711] ; Liaoning Provincial BaiQianWan Talents Program[2017-6] |
| WOS研究方向 | Marine & Freshwater Biology ; Oceanography |
| 语种 | 英语 |
| 出版者 | SCIENCE PRESS |
| WOS记录号 | WOS:000469401600010 |
| 资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances ; Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances ; Liaoning Provincial BaiQianWan Talents Program ; Liaoning Provincial BaiQianWan Talents Program ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances ; Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances ; Liaoning Provincial BaiQianWan Talents Program ; Liaoning Provincial BaiQianWan Talents Program ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances ; Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances ; Liaoning Provincial BaiQianWan Talents Program ; Liaoning Provincial BaiQianWan Talents Program ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances ; Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances ; Liaoning Provincial BaiQianWan Talents Program ; Liaoning Provincial BaiQianWan Talents Program |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/172079]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Yu Weiting; Liu Xiudong |
| 作者单位 | 1.Dalian Univ, Coll Environm & Chem Engn, Dalian 116622, Peoples R China 2.Dalian Univ, Affiliated Zhongshan Hosp, Dalian 116001, Peoples R China 3.Qingdao Brightmoon Seaweed Grp Co Ltd, State Key Lab Bioact Seaweed Subst, Qingdao 266400, Shandong, Peoples R China 4.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hou Dandan,Yu Weiting,Zhang Demeng,et al. Culture of yeast cells immobilized by alginate-chitosan microcapsules in aqueous-organic solvent biphasic system[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2019,37(3):863-870. |
| APA | Hou Dandan,Yu Weiting,Zhang Demeng,Zhao Lili,Liu Xiudong,&Ma Xiaojun.(2019).Culture of yeast cells immobilized by alginate-chitosan microcapsules in aqueous-organic solvent biphasic system.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,37(3),863-870. |
| MLA | Hou Dandan,et al."Culture of yeast cells immobilized by alginate-chitosan microcapsules in aqueous-organic solvent biphasic system".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 37.3(2019):863-870. |
入库方式： OAI收割
来源：大连化学物理研究所
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