An overview on enrichment methods for cell surface proteome profiling
文献类型：期刊论文
| 作者 | Li, Yanan1,2; Qin, Hongqiang1; Ye, Mingliang1 |
| 刊名 | JOURNAL OF SEPARATION SCIENCE
 |
| 出版日期 | 2019-09-30 |
| 页码 | 21 |
| ISSN号 | 1615-9306 |
| 关键词 | cell surface proteins enrichment methods plasma membrane proteins proteomics |
| DOI | 10.1002/jssc.201900700 |
| 通讯作者 | Ye, Mingliang(mingliang@dicp.ac.cn) |
| 英文摘要 | Cell surface proteins are essential for many important biological processes, including cell-cell interactions, signal transduction, and molecular transportation. With the characteristics of low abundance, high hydrophobicity, and high heterogeneity, it is difficult to get a comprehensive view of cell surface proteome by direct analysis. Thus, it is important to selectively enrich the cell surface proteins before liquid chromatography with mass spectrometry analysis. In recent years, a variety of enrichment methods have been developed. Based on the separation mechanism, these methods could be mainly classified into three types. The first type is based on their difference in the physicochemical property, such as size, density, charge, and hydrophobicity. The second one is based on the bimolecular affinity interaction with lectin or antibody. And the third type is based on the chemical covalent coupling to free side groups of surface-exposed proteins or carbohydrate chains, such as primary amines, carboxyl groups, glycan side chains. In addition, metabolic labeling and enzymatic reaction-based methods have also been employed to selectively isolate cell surface proteins. In this review, we will provide a comprehensive overview of the enrichment methods for cell surface proteome profiling. |
| WOS关键词 | PLASMA-MEMBRANE PROTEINS ; FREE-FLOW ELECTROPHORESIS ; ENZYME-MEDIATED ACTIVATION ; EMBRYONIC STEM-CELLS ; MASS-SPECTROMETRY ; LECTIN AFFINITY ; IN-VIVO ; CLICK CHEMISTRY ; N-GLYCOPROTEOME ; LIVING CELLS |
| 资助项目 | National Natural Science Foundation of China[81430072] ; National Natural Science Foundation of China[21535008] ; National Natural Science Foundation of China[91753105] ; China State Key Basic Research Program[2016YFA0501402] ; China State Key Basic Research Program[2017YFA0505004] ; China State Key Basic Research Program[2018YFC0910302] ; innovation program of science and research from the DICP[DICP TMSR201601] ; innovation program of science and research from the DICP[DICP& QIBEBT UN201802] ; Innovation Academy for Precision Measurement Science and Technology, CAS ; LiaoNing Revitalization Talents Program ; National Science Fund of China for Distinguished Young Scholars[21525524] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| WOS记录号 | WOS:000488151300001 |
| 资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; China State Key Basic Research Program ; China State Key Basic Research Program ; innovation program of science and research from the DICP ; innovation program of science and research from the DICP ; Innovation Academy for Precision Measurement Science and Technology, CAS ; Innovation Academy for Precision Measurement Science and Technology, CAS ; LiaoNing Revitalization Talents Program ; LiaoNing Revitalization Talents Program ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China State Key Basic Research Program ; China State Key Basic Research Program ; innovation program of science and research from the DICP ; innovation program of science and research from the DICP ; Innovation Academy for Precision Measurement Science and Technology, CAS ; Innovation Academy for Precision Measurement Science and Technology, CAS ; LiaoNing Revitalization Talents Program ; LiaoNing Revitalization Talents Program ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China State Key Basic Research Program ; China State Key Basic Research Program ; innovation program of science and research from the DICP ; innovation program of science and research from the DICP ; Innovation Academy for Precision Measurement Science and Technology, CAS ; Innovation Academy for Precision Measurement Science and Technology, CAS ; LiaoNing Revitalization Talents Program ; LiaoNing Revitalization Talents Program ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China State Key Basic Research Program ; China State Key Basic Research Program ; innovation program of science and research from the DICP ; innovation program of science and research from the DICP ; Innovation Academy for Precision Measurement Science and Technology, CAS ; Innovation Academy for Precision Measurement Science and Technology, CAS ; LiaoNing Revitalization Talents Program ; LiaoNing Revitalization Talents Program ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/172487]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Ye, Mingliang |
| 作者单位 | 1.Chinese Acad Sci, CAS Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Yanan,Qin, Hongqiang,Ye, Mingliang. An overview on enrichment methods for cell surface proteome profiling[J]. JOURNAL OF SEPARATION SCIENCE,2019:21. |
| APA | Li, Yanan,Qin, Hongqiang,&Ye, Mingliang.(2019).An overview on enrichment methods for cell surface proteome profiling.JOURNAL OF SEPARATION SCIENCE,21. |
| MLA | Li, Yanan,et al."An overview on enrichment methods for cell surface proteome profiling".JOURNAL OF SEPARATION SCIENCE (2019):21. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。