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Purification of tertiary and quaternary alkaloids from Rhizoma Corydalis using reversed-phase/weak cation-exchange mixed-mode class separation combined with preparative C18 and silica based strong cation-exchange chromatography

文献类型：期刊论文

作者Guo, Xiujie1,2,4; Gao, Zhenhua1,3; Wang, Jixia1,2; Guo, Zhimou1,2; Zhang, Xiuli2; Liu, Yanfang1,2; Wang, Lianzhi3; Wang, Chaoran1,2; Liang, Xinmiao1,2
刊名JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
出版日期2019-09-15
卷号1126页码:7
关键词Alkaloids Purification C18WCx Scx Rhizoma Corydalis
ISSN号1570-0232
DOI10.1016/j.jchromb.2019.121742
通讯作者Wang, Chaoran(wcrpj_505@dicp.ac.cn) ; Liang, Xinmiao(liangxm@dicp.ac.cn)
英文摘要A new optimization strategy for purification of alkaloids from Rhizoma Corydalis using preparative liquid chromatography was developed, featuring a selective separation of different types of alkaloids into different parts by a reversed-phase/weak cation-exchange mixed-mode column (named C18WCX) at first. The total alkaloids of Rhizoma Corydalis were divided into four fractions with fraction III and IV corresponding to the tertiary type medium bases and the quaternary type strong bases, respectively. For fraction III, a conventional C18 column was used to isolate tertiary alkaloids using acetonitrile and 0.1% phosphoric acid (adjusted with triethylamine to pH 6.0) as mobile phases. High selectivity and symmetrical peak shapes of tertiary alkaloids were obtained, resulting in six main tertiary alkaloids isolated in a single run. As strong bases, quaternary alkaloids often suffer from serious peak tailing problem on conventional C18 columns. Therefore, a silica-based strong cation-exchange (SCX) column was used for purification of fraction IV. On the SCX column, good peak shapes in high sample loading were achieved. Five main quaternary alkaloids were isolated and identified from the fraction in one-step. The procedures presented effective for the preparative isolation and purification of alkaloids from Rhizoma Corydalis.
WOS关键词TRADITIONAL CHINESE MEDICINE ; W. T. WANG ; DL-TETRAHYDROPALMATINE ; YANHUSUO WANG,W.T. ; BASIC COMPOUNDS ; PERFORMANCE ; PLANT
资助项目DICP, CAS[DICP 1201917] ; DICP, CAS[ZZBS201803] ; DICP-CMC Innovation Institute of Medicine
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
WOS记录号WOS:000488136700006
出版者ELSEVIER
资助机构DICP, CAS ; DICP, CAS ; DICP-CMC Innovation Institute of Medicine ; DICP-CMC Innovation Institute of Medicine ; DICP, CAS ; DICP, CAS ; DICP-CMC Innovation Institute of Medicine ; DICP-CMC Innovation Institute of Medicine ; DICP, CAS ; DICP, CAS ; DICP-CMC Innovation Institute of Medicine ; DICP-CMC Innovation Institute of Medicine ; DICP, CAS ; DICP, CAS ; DICP-CMC Innovation Institute of Medicine ; DICP-CMC Innovation Institute of Medicine
源URL[http://cas-ir.dicp.ac.cn/handle/321008/172514]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者Wang, Chaoran; Liang, Xinmiao
作者单位1.DICP CMC Innovat Inst Med, Taizhou 225300, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.Hubei Minzu Univ, Sch Chem & Environm Engn, Enshi 445000, Peoples R China
4.Taizhou New Drug Innovat Serv Ctr, Taizhou 225300, Peoples R China
推荐引用方式
GB/T 7714		 Guo, Xiujie,Gao, Zhenhua,Wang, Jixia,et al. Purification of tertiary and quaternary alkaloids from Rhizoma Corydalis using reversed-phase/weak cation-exchange mixed-mode class separation combined with preparative C18 and silica based strong cation-exchange chromatography[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2019,1126:7.
APA Guo, Xiujie.,Gao, Zhenhua.,Wang, Jixia.,Guo, Zhimou.,Zhang, Xiuli.,...&Liang, Xinmiao.(2019).Purification of tertiary and quaternary alkaloids from Rhizoma Corydalis using reversed-phase/weak cation-exchange mixed-mode class separation combined with preparative C18 and silica based strong cation-exchange chromatography.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1126,7.
MLA Guo, Xiujie,et al."Purification of tertiary and quaternary alkaloids from Rhizoma Corydalis using reversed-phase/weak cation-exchange mixed-mode class separation combined with preparative C18 and silica based strong cation-exchange chromatography".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1126(2019):7.

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来源：大连化学物理研究所

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