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Spectroscopic approach for the interaction of carbon nanoparticles with cytochrome c and BY-2 cells: Protein structure and mitochondrial function

文献类型：期刊论文

作者Chen, Lijuan2,3,4; Hao, Jufang5; Xu, Liangtao1; Meng, Xiangyu1; Li, Xiang1; Nie, Cong1; Xie, Fuwei1; Liu, Kejian1; Peng, Xiaojun2; Xie, Jianping1
刊名INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
出版日期2019-10-01
卷号138页码:29-36
关键词Carbon nanopartides Cytochrome c Conformation Redox potential Mitochondrial function
ISSN号0141-8130
DOI10.1016/j.ijbiomac.2019.07.076
通讯作者Xie, Jianping(ztridicp@126.com)
英文摘要In this study, we employed multiple spectroscopic methods to analyze the effects of carbon nanoparticles (CNPs) on structure of cytochrome c (Cyt c) and mitochondrial function in plant cells. The tertiary structures of aromatic amino acid in Cyt c were not changed after addition of CNPs. Cyt c was found to be absorbed on the surfaces of CNPs in a non-linear manner and only bound Cyt c can be reduced. In addition, the binding of Cyt c was found to increase the diameter of CNPs at lower concentrations. The redox potential of Cyt c was almost not affected after treatment with CNPs. There were no obvious differences in cellular ATP after exposure to CNPs, and the mitochondrial membrane potential (MMP) was significantly decreased once the CNPs concentration exceeded 31.25 mu g/mL. The levels of reactive oxygen species (ROS) also were increased in BY-2 cells. Taken together, these findings provide basis for the interactions between CNPs and Cyt c, as well as the effect of CNPs treatment on the mitochondria function in plant cells. (C) 2019 Published by Elsevier B.V.
WOS关键词SILICA NANOPARTICLES ; INDUCED APOPTOSIS ; GRAPHENE OXIDE ; NANOTUBES ; ACTIVATION ; STABILITY ; KINETICS
资助项目College of Chemistry and Molecular Engineering, Zhengzhou University
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种英语
WOS记录号WOS:000487569000004
出版者ELSEVIER
资助机构College of Chemistry and Molecular Engineering, Zhengzhou University ; College of Chemistry and Molecular Engineering, Zhengzhou University ; College of Chemistry and Molecular Engineering, Zhengzhou University ; College of Chemistry and Molecular Engineering, Zhengzhou University ; College of Chemistry and Molecular Engineering, Zhengzhou University ; College of Chemistry and Molecular Engineering, Zhengzhou University ; College of Chemistry and Molecular Engineering, Zhengzhou University ; College of Chemistry and Molecular Engineering, Zhengzhou University
源URL[http://cas-ir.dicp.ac.cn/handle/321008/172560]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者Xie, Jianping
作者单位1.Zhengzhou Tobacco Res Inst CNTC, 2 Fengyang Rd, Zhengzhou 450001, Henan, Peoples R China
2.Dalian Univ Technol, State Key Lab Fine Chem, 2 Linggong Rd, Dalian 116024, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Peoples R China
4.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China
5.Staff Dev Inst China Natl Tobacco Corp, 7 Xinyuan Rd, Zhengzhou 450008, Henan, Peoples R China
6.Beijing Xinna Int Hitech Mat Co Ltd, 14 Kechuang St, Beijing 100076, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Lijuan,Hao, Jufang,Xu, Liangtao,et al. Spectroscopic approach for the interaction of carbon nanoparticles with cytochrome c and BY-2 cells: Protein structure and mitochondrial function[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2019,138:29-36.
APA Chen, Lijuan.,Hao, Jufang.,Xu, Liangtao.,Meng, Xiangyu.,Li, Xiang.,...&Guo, Zhijiang.(2019).Spectroscopic approach for the interaction of carbon nanoparticles with cytochrome c and BY-2 cells: Protein structure and mitochondrial function.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,138,29-36.
MLA Chen, Lijuan,et al."Spectroscopic approach for the interaction of carbon nanoparticles with cytochrome c and BY-2 cells: Protein structure and mitochondrial function".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 138(2019):29-36.

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来源：大连化学物理研究所

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