Simple fabrication of inner chitosan-coated alginate hollow microfiber with higher stability
文献类型：期刊论文
| 作者 | Liu, Hui1; Wang, Yaqing1,2; Yu, Yue1; Chen, Wenwen1,2; Jiang, Lei1; Qin, Jianhua1,2,3,4 |
| 刊名 | JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
 |
| 出版日期 | 2019-11-01 |
| 卷号 | 107期号:8页码:2527-2536 |
| 关键词 | microfluidics alginate microfibers chitosan coating microfiber stability cell culture |
| ISSN号 | 1552-4973 |
| DOI | 10.1002/jbm.b.34343 |
| 通讯作者 | Qin, Jianhua(jhqin@dicp.ac.cn) |
| 英文摘要 | Sodium alginate (NaA) has been widely used as microfiber-based scaffold material. However, Ca-alginate microfiber might disintegrate in the physiological environment due to the loss of calcium ions, which will limit its long-term application in tissue engineering. In this work, to enhance the stability of Ca-alginate microfiber in the physiological environment, an inner chitosan coating was introduced to Ca-alginate hollow microfiber by one step via a microfluidic device. A more stable composite microfiber with double cross-linking layers was generated. The stability of the microfiber was studied in the PBS solution (pH 7.4) to identify the coating effect on the hollow structure. The results revealed that chitosan component bonded an NaA layer to form a stable polyelectrolyte complex membrane in the inner wall of the microfiber, which stabilized the hollow region even though the Ca-alginate shell was disintegrated by PBS solution. In addition, the introduction of chitosan coating improved the inner environment of the low affinity of alginate to cell surfaces and facilitated the cell adhesion and culture in the microfiber. HepG2 cells in the microfibers displayed favorable cell viability and proliferation ability. We believe that this work will lead to the development of innovative methodologies and materials for both cell culture and tissue engineering application. (c) 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B:2527-2536, 2019. |
| WOS关键词 | CELL-LADEN MICROFIBERS ; FIBERS ; HYDROGELS ; NANOPARTICLES ; SCAFFOLDS ; DELIVERY ; MATRIX |
| 资助项目 | National Nature Science Foundation of China[31600784] ; National Nature Science Foundation of China[31671038] ; National Nature Science Foundation of China[81573394] ; National Nature Science Foundation of China[81603075] ; National Nature Science Foundation of China[91543121] ; Key Program of the Chinese Academy of Sciences[KFZD-SW-213] ; National Key R&D Program of China[2017YFB0405400] ; Innovation Program of Science and Research from the DICP, CAS[TMSR201601] ; Innovation Program of Science and Research from the DICP, CAS[DICP TMSR201601] |
| WOS研究方向 | Engineering ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000488626300008 |
| 出版者 | WILEY |
| 资助机构 | National Nature Science Foundation of China ; National Nature Science Foundation of China ; Key Program of the Chinese Academy of Sciences ; Key Program of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; Key Program of the Chinese Academy of Sciences ; Key Program of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; Key Program of the Chinese Academy of Sciences ; Key Program of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; Key Program of the Chinese Academy of Sciences ; Key Program of the Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/172575]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Qin, Jianhua |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, CAS Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Stem Cell & Regenerat, Beijing, Peoples R China 4.Chinese Acad Sci, CAS Ctr Excellence Brain Sci & Intelligence Techn, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Hui,Wang, Yaqing,Yu, Yue,et al. Simple fabrication of inner chitosan-coated alginate hollow microfiber with higher stability[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS,2019,107(8):2527-2536. |
| APA | Liu, Hui,Wang, Yaqing,Yu, Yue,Chen, Wenwen,Jiang, Lei,&Qin, Jianhua.(2019).Simple fabrication of inner chitosan-coated alginate hollow microfiber with higher stability.JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS,107(8),2527-2536. |
| MLA | Liu, Hui,et al."Simple fabrication of inner chitosan-coated alginate hollow microfiber with higher stability".JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 107.8(2019):2527-2536. |
入库方式： OAI收割
来源：大连化学物理研究所
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