Two-stage fermentation optimization for poly-3-hydroxybutyrate production from methanol by a new Methylobacterium isolate from oil fields
文献类型：期刊论文
| 作者 | Wang, J.1,2; Tan, H.2; Li, K.2; Yin, H.2 |
| 刊名 | JOURNAL OF APPLIED MICROBIOLOGY
 |
| 出版日期 | 2019-10-18 |
| 页码 | 11 |
| ISSN号 | 1364-5072 |
| 关键词 | Methylobacterium sp 1805 oil fields polyhydroxybutyrate response surface methodology two-stage fermentation |
| DOI | 10.1111/jam.14463 |
| 通讯作者 | Yin, H.(yinheng@dicp.ac.cn) |
| 英文摘要 | Aims We aimed to explore a new Methylobacterium isolate to produce polyhydroxybutyrate (PHB) by using methanol as a sole carbon resource and improve PHB production. Methods and Results A new PHB-producing isolate (Methylobacterium sp. 1805) was obtained from oil fields by using methanol as a sole carbon source. The fermentation situation of PHB production was further optimized by using Box-Behnken response surface methodology (RSM). Before optimization, the cell biomass was 0 center dot 6 g l(-1) after 3-day culture and 0 center dot 3 g l(-1) PHB was produced after 5-day methanol-inducing stage. The RSM growth medium was optimized as 15 g l(-1) glycerol, 10 g l(-1) beef extract and 0 center dot 65 g l(-1) MgSO4 center dot 7H(2)O. The RSM methanol-inducing medium was optimized as 0 center dot 65 g l(-1) MgSO4 (metal ions), 20 mmol l(-1) PBS pH 6 center dot 5 and final 2% methanol (v/v). The biomass and PHB production reached 1 center dot 0 and 0 center dot 55 g l(-1) after 3-day culture, respectively. The PHB yield increased by about 80% when compared with before optimization. Conclusions The optimization of a two-stage fermentation process improved PHB production from methanol by using Methylobacterium sp. 1805. Significance and Impact of the Study A new Methylobacterium isolate was isolated and produced high-level PHB by using methanol as a sole carbon resource. The bacteria will provide a potential tool for C1 resource in producing PHB. |
| WOS关键词 | POLY-BETA-HYDROXYBUTYRATE ; PHA ; BIOSYNTHESIS ; EXTORQUENS ; SYNTHASE ; WATER ; CONSORTIUM |
| 资助项目 | Chinese National Nature Science Foundation[31670803] ; Chinese National Nature Science Foundation[31770847] ; DICP[ZZBS201704] ; DICP[BioChE-X201801] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Microbiology |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:000490873500001 |
| 资助机构 | Chinese National Nature Science Foundation ; Chinese National Nature Science Foundation ; DICP ; DICP ; Chinese National Nature Science Foundation ; Chinese National Nature Science Foundation ; DICP ; DICP ; Chinese National Nature Science Foundation ; Chinese National Nature Science Foundation ; DICP ; DICP ; Chinese National Nature Science Foundation ; Chinese National Nature Science Foundation ; DICP ; DICP |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/172581]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Yin, H. |
| 作者单位 | 1.Dalian Ocean Univ, Coll Fisheries & Life Sci, Dalian, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Liaoning Prov Key Lab Carbohydrates, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, J.,Tan, H.,Li, K.,et al. Two-stage fermentation optimization for poly-3-hydroxybutyrate production from methanol by a new Methylobacterium isolate from oil fields[J]. JOURNAL OF APPLIED MICROBIOLOGY,2019:11. |
| APA | Wang, J.,Tan, H.,Li, K.,&Yin, H..(2019).Two-stage fermentation optimization for poly-3-hydroxybutyrate production from methanol by a new Methylobacterium isolate from oil fields.JOURNAL OF APPLIED MICROBIOLOGY,11. |
| MLA | Wang, J.,et al."Two-stage fermentation optimization for poly-3-hydroxybutyrate production from methanol by a new Methylobacterium isolate from oil fields".JOURNAL OF APPLIED MICROBIOLOGY (2019):11. |
入库方式： OAI收割
来源：大连化学物理研究所
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