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A digital microfluidic diluter-based microalgal motion biosensor for marine pollution monitoring

文献类型：期刊论文

作者Han, Shuang2,6; Zhang, Qian2,6; Zhang, Xingcai3; Liu, Xianming4,6; Lu, Ling1; Wei, Junfeng2,6; Li, Yuancheng5; Wang, Yunhua1,6; Zheng, Guoxia2,6
刊名BIOSENSORS & BIOELECTRONICS
出版日期2019-10-15
卷号143页码:5
ISSN号0956-5663
关键词Algae biosensor Motility signal Digital microfluidics Marine monitoring Feedback control Cross-split
DOI10.1016/j.bios.2019.111597
通讯作者Li, Yuancheng(yuancheng1999@163.com) ; Wang, Yunhua(yunhua1977@126.com) ; Zheng, Guoxia(guoxiazheng2685@163.com)
英文摘要Marine pollution and monitoring have received more and more concern in recent years. Herein, a fully automatic whole-algae biosensor was designed for low-cost and fast detection of toxic contaminants in seawater. It consists of a digital microfluidic (DMF) diluter chip, an actuation element, a detector element, and a microalgae bioreporter. A feedback-control protocol based on charging-time compensation was introduced. It ensures precise actuation of the droplet with diverse salty concentrations and contents in the marine environment. The two mixer cross-split dilution engine increases the accuracy of droplet dispensing and concentration diluting. By selecting motility of P. subcordiformis as the sensor signal, the developed biosensor showed good sensitivity and robustness for a wide range of salinity (10-37 parts per thousand), temperature (0-25 degrees C), light levels (0-325 mu mol photons m(-2) s(-1)), and cell density factor (1.0-4.0). The biosensor responses were examined in the presence of copper, lead, phenol, and nonylphenol (NP). In all cases, toxic responses (i.e. dose-related inhibition of algal motion) were detected with the detection limits of 0.65 mu mol.L-1, 1.90 mu mol.L-1, 2.85 mmol.L-1, and 5.22 mu mol.l(-1) respectively. These results were obtained in a much shorter time (2 h for our biosensor vs. 24 h-10 d for growth inhibition test) and the data are consistent with previous classical studies. We thus developed a simple, rapid, and adaptable system for marine routine monitoring and early-warning detection for lab and on-site applications.
资助项目National Natural Science Foundation of China[41476085] ; National Natural Science Foundation of China[81471807] ; Scientific Research Project of Liaoning Education Department[LJQ 2015005]
WOS研究方向Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
语种英语
出版者ELSEVIER ADVANCED TECHNOLOGY
WOS记录号WOS:000487765000035
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; Scientific Research Project of Liaoning Education Department ; Scientific Research Project of Liaoning Education Department ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Scientific Research Project of Liaoning Education Department ; Scientific Research Project of Liaoning Education Department ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Scientific Research Project of Liaoning Education Department ; Scientific Research Project of Liaoning Education Department ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Scientific Research Project of Liaoning Education Department ; Scientific Research Project of Liaoning Education Department
源URL[http://cas-ir.dicp.ac.cn/handle/321008/172627]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者Li, Yuancheng; Wang, Yunhua; Zheng, Guoxia
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Dalian Univ, Environm & Chem Engn Inst, Dalian 116622, Peoples R China
3.Harvard Univ, John A Paulson Sch Engn & Appl Sci, Cambridge, MA 02138 USA
4.Dalian Univ, Med Sch, Dalian 116622, Peoples R China
5.Dalian Med Univ, Affiliated Hosp 1, Dalian 116001, Peoples R China
6.Dalian Univ, Environm Micro Total Anal Lab, Dalian 116622, Peoples R China
推荐引用方式
GB/T 7714		 Han, Shuang,Zhang, Qian,Zhang, Xingcai,et al. A digital microfluidic diluter-based microalgal motion biosensor for marine pollution monitoring[J]. BIOSENSORS & BIOELECTRONICS,2019,143:5.
APA Han, Shuang.,Zhang, Qian.,Zhang, Xingcai.,Liu, Xianming.,Lu, Ling.,...&Zheng, Guoxia.(2019).A digital microfluidic diluter-based microalgal motion biosensor for marine pollution monitoring.BIOSENSORS & BIOELECTRONICS,143,5.
MLA Han, Shuang,et al."A digital microfluidic diluter-based microalgal motion biosensor for marine pollution monitoring".BIOSENSORS & BIOELECTRONICS 143(2019):5.

入库方式： OAI收割

来源：大连化学物理研究所

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