Human Milk Oligosaccharides Protect against Necrotizing Enterocolitis by Inhibiting Intestinal Damage via Increasing the Proliferation of Crypt Cells
文献类型：期刊论文
| 作者 | Wang, Chenyuan2,3; Zhang, Ming1; Guo, Huiyuan2,3; Yan, Jingyu4; Liu, Fan2; Chen, Jianliang3; Li, Yiran3; Ren, Fazheng2,3 |
| 刊名 | MOLECULAR NUTRITION & FOOD RESEARCH
 |
| 出版日期 | 2019-09-01 |
| 卷号 | 63期号:18页码:12 |
| ISSN号 | 1613-4125 |
| 关键词 | crypt organoid human milk oligosaccharides inflammation necrotizing enterocolitis toll-like receptor 4 nuclear factor kappa-B pathway |
| DOI | 10.1002/mnfr.201900262 |
| 通讯作者 | Ren, Fazheng(renfazheng@cau.edu.cn) |
| 英文摘要 | Scope Necrotizing enterocolitis (NEC) is a devastating disease that is highly lethal in premature infants. Human milk oligosaccharides (HMOs) efficiently reduce the incidence of NEC. However, the protective mechanism of HMO treatment is unknown. It is hypothesized that HMOs protect against NEC by inhibiting the damage to intestinal epithelial cells. Methods and results C57BL/6 pups are challenged with hypoxia and cold stress to induce NEC. All pups are sacrificed after 72 h. It is found that HMO administration reduces the concentrations of IL-8 in the serum and ileum of all NEC mice. Ileum toll-like receptor 4 (TLR4) protein expression and nuclear factor kappa-B (NF kappa B) pathway activation are inhibited. The proliferative ability of enterocytes in the ileum is restored as determined by labeling with proliferation markers (Ki67, SOX9). In a 3D culture intestinal crypt organoids study, HMO treatment improves the maturation of organoid cells and increases the ratio of proliferative cells under lipopolysaccharides (LPS) treatment. HMO treatment downregulates TLR4 expression in the organoid cells, thus reducing the effect of LPS. Conclusion HMOs protect intestinal epithelial cells from injury by accelerating the turnover of crypt cells by reducing the expression of TLR4 on intestinal epithelial cells. |
| WOS关键词 | NF-KAPPA-B ; STEM-CELLS ; RECEPTOR 4 ; RAT MODEL ; TLR4 ; PATHOGENESIS ; EXPRESSION ; NEWBORNS ; INJURY |
| WOS研究方向 | Food Science & Technology |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:000487033600012 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/172674]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Ren, Fazheng |
| 作者单位 | 1.Beijing Technol & Business Univ, Sch Food & Chem Engn, Beijing 100048, Peoples R China 2.China Agr Univ, Coll Food Sci & Nutr Engn, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Beijing 100083, Peoples R China 3.China Agr Univ, Key Lab Funct Dairy, Beijing 100083, Peoples R China 4.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Chenyuan,Zhang, Ming,Guo, Huiyuan,et al. Human Milk Oligosaccharides Protect against Necrotizing Enterocolitis by Inhibiting Intestinal Damage via Increasing the Proliferation of Crypt Cells[J]. MOLECULAR NUTRITION & FOOD RESEARCH,2019,63(18):12. |
| APA | Wang, Chenyuan.,Zhang, Ming.,Guo, Huiyuan.,Yan, Jingyu.,Liu, Fan.,...&Ren, Fazheng.(2019).Human Milk Oligosaccharides Protect against Necrotizing Enterocolitis by Inhibiting Intestinal Damage via Increasing the Proliferation of Crypt Cells.MOLECULAR NUTRITION & FOOD RESEARCH,63(18),12. |
| MLA | Wang, Chenyuan,et al."Human Milk Oligosaccharides Protect against Necrotizing Enterocolitis by Inhibiting Intestinal Damage via Increasing the Proliferation of Crypt Cells".MOLECULAR NUTRITION & FOOD RESEARCH 63.18(2019):12. |
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来源：大连化学物理研究所
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