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Stereostructural Elucidation of Glucose Phosphorylation by Raman Optical Activity

文献类型：期刊论文

作者Tang, Yuxuan1,2; Cheng, Feng1; Feng, Zhaochi1; Jia, Guoqing1; Li, Can1
刊名JOURNAL OF PHYSICAL CHEMISTRY B
出版日期2019-09-19
卷号123期号:37页码:7794-7800
ISSN号1520-6106
DOI10.1021/acs.jpcb.9b05968
通讯作者Jia, Guoqing(gqjia@dicp.ac.cn) ; Li, Can(canli@dicp.ac.cn)
英文摘要Phosphorylation of glucose is the prime step in sugar metabolism and energy storage. Two key glucose phosphates are involved, that is, glucose 6-phosphate (G6P) and alpha-glucose 1-phosphate (alpha G1P). The chiral conformation of glucose, G6P, and alpha G1P plays an essential role in enzyme-mediated conversions. However, few techniques were able to give a direct view of the conformational changes from glucose to G6P and alpha G1P. Here, Raman optical activity (ROA) was used to elucidate the stereochemical evolution of glucose upon phosphorylation. ROA was found to be extremely sensitive to different phosphorylation sites. A characteristic ROA marker of (+)980 cm(-1) originated from the phosphate group symmetric stretching vibration, is observed for alpha G1P with phosphorylation at chiral C1, while Phosphorylation-induced gauch-gauch (gg)/gauch-trans (gt) rotamer distribution changes no corresponding ROA signal for G6P with phosphorylation at achiral C6 is observed. can be sensitively probed by the sign of the ROA band around 1460 cm(-1). A positive ROA band at 1465 cm(-1) of glucose corresponds to a higher gt ratio, while a negative band at 1455 cm(-1) of G6P suggests a dominant gg population, and the disappearance of this ROA band for alpha G1P indicates a nearly balanced gg/gt distribution. Meanwhile, the phosphorylation at C6 and C1 could cause dramatic reduction of the conformational flexibility of the adjacent C4-OH and C2-OH, respectively. These stereochemical changes revealed by ROA spectra offer a structural basis on the understanding of sugar phosphorylation from the perspective of chirality.
WOS关键词MOLECULAR-DYNAMICS ; ANOMERIC SPECIFICITY ; ACTIVITY SPECTRA ; SPECTROSCOPY ; SIMULATION ; GLUCOKINASE ; GROMACS ; QUANTUM ; ACID ; RECOGNITION
资助项目National Major Scientific Instruments and Equipment Development Project[21227801] ; National Natural Science Foundation of China[21173213] ; Instrument Developing Project of the Chinese Academy of Sciences
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000487179700004
出版者AMER CHEMICAL SOC
资助机构National Major Scientific Instruments and Equipment Development Project ; National Major Scientific Instruments and Equipment Development Project ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; National Major Scientific Instruments and Equipment Development Project ; National Major Scientific Instruments and Equipment Development Project ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; National Major Scientific Instruments and Equipment Development Project ; National Major Scientific Instruments and Equipment Development Project ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; National Major Scientific Instruments and Equipment Development Project ; National Major Scientific Instruments and Equipment Development Project ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences
源URL[http://cas-ir.dicp.ac.cn/handle/321008/172706]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者Jia, Guoqing; Li, Can
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Zhongshan Rd 457, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Tang, Yuxuan,Cheng, Feng,Feng, Zhaochi,et al. Stereostructural Elucidation of Glucose Phosphorylation by Raman Optical Activity[J]. JOURNAL OF PHYSICAL CHEMISTRY B,2019,123(37):7794-7800.
APA Tang, Yuxuan,Cheng, Feng,Feng, Zhaochi,Jia, Guoqing,&Li, Can.(2019).Stereostructural Elucidation of Glucose Phosphorylation by Raman Optical Activity.JOURNAL OF PHYSICAL CHEMISTRY B,123(37),7794-7800.
MLA Tang, Yuxuan,et al."Stereostructural Elucidation of Glucose Phosphorylation by Raman Optical Activity".JOURNAL OF PHYSICAL CHEMISTRY B 123.37(2019):7794-7800.

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来源：大连化学物理研究所

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