Challenges and Advances in the Fabrication of Monolithic Bioseparation Materials and their Applications in Proteomics Research
文献类型：期刊论文
| 作者 | Ma, Shujuan2; Li, Ya2; Ma, Chen2; Wang, Yan2; Ou, Junjie1,2; Ye, Mingliang1,2 |
| 刊名 | ADVANCED MATERIALS
 |
| 出版日期 | 2019-09-10 |
| 页码 | 27 |
| 关键词 | affinity chromatography bioseparation glycoproteomics hybrid monoliths monolithic materials phosphoproteomics |
| ISSN号 | 0935-9648 |
| DOI | 10.1002/adma.201902023 |
| 通讯作者 | Ou, Junjie(junjieou@dicp.ac.cn) |
| 英文摘要 | High-performance liquid chromatography integrated with tandem mass spectrometry (HPLC-MS/MS) has become a powerful technique for proteomics research. Its performance heavily depends on the separation efficiency of HPLC, which in turn depends on the chromatographic material. As the "heart" of the HPLC system, the chromatographic material is required to achieve excellent column efficiency and fast analysis. Monolithic materials, fabricated as continuous supports with interconnected skeletal structure and flow-through pores, are regarded as an alternative to particle-packed columns. Such materials are featured with easy preparation, fast mass transfer, high porosity, low back pressure, and miniaturization, and are next-generation separation materials for high-throughput proteins and peptides analysis. Herein, the recent progress regarding the fabrication of various monolithic materials is reviewed. Special emphasis is placed on studies of the fabrication of monolithic capillary columns and their applications in separation of biomolecules by capillary liquid chromatography (cLC). The applications of monolithic materials in the digestion, enrichment, and separation of phosphopeptides and glycopeptides from biological samples are also considered. Finally, advances in comprehensive 2D HPLC separations using monolithic columns are also shown. |
| WOS关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY ; POROUS POLYMER MONOLITHS ; POLYHEDRAL OLIGOMERIC SILSESQUIOXANE ; POLAR STATIONARY PHASES ; ONE-POT PREPARATION ; LARGE SURFACE-AREA ; LECTIN AFFINITY-CHROMATOGRAPHY ; HIGHLY EFFICIENT SEPARATION ; IMMOBILIZED ENZYME REACTORS ; ENE CLICK REACTION |
| 资助项目 | China State Key Basic Research Program[2017YFA0505004] ; China State Key Basic Research Program[2016YFA0501402] ; Dalian Institute of Chemical Physics, Chinese Academy of Sciences (DICP, CAS)[DICP TMSR201601] ; National Natural Sciences Foundation of China[21575141] ; CAS-Weigao Research & Development Program[[2017]-009] |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics |
| 语种 | 英语 |
| WOS记录号 | WOS:000486129600001 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 资助机构 | China State Key Basic Research Program ; China State Key Basic Research Program ; Dalian Institute of Chemical Physics, Chinese Academy of Sciences (DICP, CAS) ; Dalian Institute of Chemical Physics, Chinese Academy of Sciences (DICP, CAS) ; National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China ; CAS-Weigao Research & Development Program ; CAS-Weigao Research & Development Program ; China State Key Basic Research Program ; China State Key Basic Research Program ; Dalian Institute of Chemical Physics, Chinese Academy of Sciences (DICP, CAS) ; Dalian Institute of Chemical Physics, Chinese Academy of Sciences (DICP, CAS) ; National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China ; CAS-Weigao Research & Development Program ; CAS-Weigao Research & Development Program ; China State Key Basic Research Program ; China State Key Basic Research Program ; Dalian Institute of Chemical Physics, Chinese Academy of Sciences (DICP, CAS) ; Dalian Institute of Chemical Physics, Chinese Academy of Sciences (DICP, CAS) ; National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China ; CAS-Weigao Research & Development Program ; CAS-Weigao Research & Development Program ; China State Key Basic Research Program ; China State Key Basic Research Program ; Dalian Institute of Chemical Physics, Chinese Academy of Sciences (DICP, CAS) ; Dalian Institute of Chemical Physics, Chinese Academy of Sciences (DICP, CAS) ; National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China ; CAS-Weigao Research & Development Program ; CAS-Weigao Research & Development Program |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/173026]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Ou, Junjie |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, CAS Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Shujuan,Li, Ya,Ma, Chen,et al. Challenges and Advances in the Fabrication of Monolithic Bioseparation Materials and their Applications in Proteomics Research[J]. ADVANCED MATERIALS,2019:27. |
| APA | Ma, Shujuan,Li, Ya,Ma, Chen,Wang, Yan,Ou, Junjie,&Ye, Mingliang.(2019).Challenges and Advances in the Fabrication of Monolithic Bioseparation Materials and their Applications in Proteomics Research.ADVANCED MATERIALS,27. |
| MLA | Ma, Shujuan,et al."Challenges and Advances in the Fabrication of Monolithic Bioseparation Materials and their Applications in Proteomics Research".ADVANCED MATERIALS (2019):27. |
入库方式： OAI收割
来源：大连化学物理研究所
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