An rGQD/chitosan nanocomposite-based pH-sensitive probe: application to sensing in urease activity assays
文献类型：期刊论文
| 作者 | Shi, Fanping2; Shang, Danyi1; Wang, Zonghua2 |
| 刊名 | NEW JOURNAL OF CHEMISTRY
 |
| 出版日期 | 2019-09-14 |
| 卷号 | 43期号:34页码:13398-13407 |
| ISSN号 | 1144-0546 |
| DOI | 10.1039/c9nj03268a |
| 通讯作者 | Wang, Zonghua(wangzonghua@qdu.edu.cn) |
| 英文摘要 | Herein, we developed a pH sensing platform based on reduced graphene quantum dots (rGQDs) and chitosan (CS). CS was used as the recognition element in the self-assembly of the rGQD/CS nanocomposite because of its fascinating pH-sensitivity, induced by the protonation and deprotonation of the -NH2 group on its edge. The -NH2 was easily protonated under acidic conditions, making CS positively charged. The negatively-charged rGQDs under weak acidic conditions thus could couple with CS through electrostatic attraction, leading to fluorescence quenching. When the pH was changed to basic, the CS became negatively charged, resulting in the disassembly of the rGQD/CS system and causing the system to exhibit a turn-on fluorescence signal. The proposed pH-sensing nanocomposite was successfully applied for sensitive and reliable pH measurements from 5.0 to 9.0. This nanocomposite system was further utilized for the sensitive detection of pH changes caused by the enzymatic activity of urease, thereby proving its utility as a fluorescence turn-on sensor for urease in the field of biochemical and environmental analysis. The photoluminescence (PL) intensity of the rGQD/CS system increased as the pH increased. The increased intensity is directly related to the urease activity in the assay system. Thus, a novel fluorescence turn-on biosensor for urease based on the disassembly of the rGQD/CS composite is proposed. The system response exhibited a nearly linear relationship with urease concentration in the range of 0.05-0.75 U mL(-1). The detection limit for urease was 0.036 U mL(-1). This is the first report of this type of sensor for urease detection. When applied to real sample analysis, the present strategy exhibited satisfactory results. |
| WOS关键词 | QUANTUM DOTS ; CARBON-DOTS ; FLUORESCENCE ; NANOPARTICLES ; BIOSENSOR ; PHOTOLUMINESCENCE ; NANOCLUSTERS |
| 资助项目 | National Natural Science Foundation of China[21475071] ; National Natural Science Foundation of China[21775052] ; Taishan Scholar Program of Shandong Province[ts 201511027] ; Qingdao postdoctoral research project funding |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000483534400012 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; Taishan Scholar Program of Shandong Province ; Taishan Scholar Program of Shandong Province ; Qingdao postdoctoral research project funding ; Qingdao postdoctoral research project funding ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Taishan Scholar Program of Shandong Province ; Taishan Scholar Program of Shandong Province ; Qingdao postdoctoral research project funding ; Qingdao postdoctoral research project funding ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Taishan Scholar Program of Shandong Province ; Taishan Scholar Program of Shandong Province ; Qingdao postdoctoral research project funding ; Qingdao postdoctoral research project funding ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Taishan Scholar Program of Shandong Province ; Taishan Scholar Program of Shandong Province ; Qingdao postdoctoral research project funding ; Qingdao postdoctoral research project funding |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/173246]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Wang, Zonghua |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, 457 Zhongshan Rd, Dalian 116023, Peoples R China 2.Qingdao Univ, Shandong Sino Japanese Ctr Collaborat Res Carbon, Coll Chem & Chem Engn, Qingdao 266071, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, Fanping,Shang, Danyi,Wang, Zonghua. An rGQD/chitosan nanocomposite-based pH-sensitive probe: application to sensing in urease activity assays[J]. NEW JOURNAL OF CHEMISTRY,2019,43(34):13398-13407. |
| APA | Shi, Fanping,Shang, Danyi,&Wang, Zonghua.(2019).An rGQD/chitosan nanocomposite-based pH-sensitive probe: application to sensing in urease activity assays.NEW JOURNAL OF CHEMISTRY,43(34),13398-13407. |
| MLA | Shi, Fanping,et al."An rGQD/chitosan nanocomposite-based pH-sensitive probe: application to sensing in urease activity assays".NEW JOURNAL OF CHEMISTRY 43.34(2019):13398-13407. |
入库方式： OAI收割
来源：大连化学物理研究所
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