Highly Efficient Analysis of Glycoprotein Sialylation in Human Serum by Simultaneous Quantification of Glycosites and Site-Specific Glycoforms
文献类型：期刊论文
| 作者 | Qin, Hongqiang1; Dong, Xuefang1; Mao, Jiawei1,2; Chen, Yao1,2; Dong, Mingming1; Wang, Liming3; Guo, Zhimou1; Liang, Xinmiao1; Ye, Mingliang1 |
| 刊名 | JOURNAL OF PROTEOME RESEARCH
 |
| 出版日期 | 2019-09-01 |
| 卷号 | 18期号:9页码:3439-3446 |
| ISSN号 | 1535-3893 |
| 关键词 | sialylation human serum glycosites intact glycopeptides site-specific glycoforms |
| DOI | 10.1021/acs.jproteome.9b00332 |
| 通讯作者 | Guo, Zhimou(guozhimou@dicp.ac.cn) ; Liang, Xinmiao(lianagxm@dicp.ac.cn) ; Ye, Mingliang(mingliang@dicp.ac.cn) |
| 英文摘要 | Aberrant sialylation of glycoproteins is closely related to many malignant diseases, and analysis of sialylation has great potential to reveal the status of these diseases. However, in-depth analysis of sialylation is still challenging because of the high microheterogeneity of protein glycosylation, as well as the low abundance of sialylated glycopeptides (SGPs). Herein, an integrated strategy was fabricated for the detailed characterization of glycoprotein sialylation on the levels of glycosites and site-specific glycoforms by employing the SGP enrichment method. This strategy enabled the identification of up to 380 glycosites, as well as 414 intact glycopeptides corresponding to 383 site-specific glycoforms from only initial 6 mu L serum samples, indicating the high sensitivity of the method for the detailed analysis of glycoprotein sialylation. This strategy was further employed to the differential analysis of glycoprotein sialylation between hepatocellular carcinoma patients and control samples, leading to the quantification of 344 glycosites and 405 site-specific glycoforms, simultaneously. Among these, 43 glycosites and 55 site-specific glycoforms were found to have significant change on the glycosite and site-specific glycoform levels, respectively. Interestingly, several glycoforms attached onto the same glycosite were found with different change tendencies. This strategy was demonstrated to be a powerful tool to reveal subtle differences of the macro- and microheterogeneity of glycoprotein sialylation. |
| WOS关键词 | N-GLYCOPEPTIDES ; CANCER ; GLYCOSYLATION ; CHROMATOGRAPHY ; EXTRACTION ; ENRICHMENT ; PROTEINS ; PLATFORM ; IMPROVES ; GLYCANS |
| 资助项目 | China State Key Basic Research Program Grants[2018YFC0910302] ; China State Key Basic Research Program Grants[2016YFA0501402] ; National Natural Science Foundation of China[21535008] ; National Natural Science Foundation of China[81430072] ; National Natural Science Foundation of China[81361128] ; National Natural Science Foundation of China[21775146] ; LiaoNing Revitalization Talents Program from the Liaoning province of China ; innovation program of Dalian Institute of Chemical Physics, CAS[DICP TMSR201601] ; innovation program of Dalian Institute of Chemical Physics, CAS[DICPQIBEBT UN201802] ; National Science Fund of China for Distinguished Young Scholars[21525524] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| 出版者 | AMER CHEMICAL SOC |
| WOS记录号 | WOS:000485089100020 |
| 资助机构 | China State Key Basic Research Program Grants ; China State Key Basic Research Program Grants ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; LiaoNing Revitalization Talents Program from the Liaoning province of China ; LiaoNing Revitalization Talents Program from the Liaoning province of China ; innovation program of Dalian Institute of Chemical Physics, CAS ; innovation program of Dalian Institute of Chemical Physics, CAS ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars ; China State Key Basic Research Program Grants ; China State Key Basic Research Program Grants ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; LiaoNing Revitalization Talents Program from the Liaoning province of China ; LiaoNing Revitalization Talents Program from the Liaoning province of China ; innovation program of Dalian Institute of Chemical Physics, CAS ; innovation program of Dalian Institute of Chemical Physics, CAS ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars ; China State Key Basic Research Program Grants ; China State Key Basic Research Program Grants ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; LiaoNing Revitalization Talents Program from the Liaoning province of China ; LiaoNing Revitalization Talents Program from the Liaoning province of China ; innovation program of Dalian Institute of Chemical Physics, CAS ; innovation program of Dalian Institute of Chemical Physics, CAS ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars ; China State Key Basic Research Program Grants ; China State Key Basic Research Program Grants ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; LiaoNing Revitalization Talents Program from the Liaoning province of China ; LiaoNing Revitalization Talents Program from the Liaoning province of China ; innovation program of Dalian Institute of Chemical Physics, CAS ; innovation program of Dalian Institute of Chemical Physics, CAS ; National Science Fund of China for Distinguished Young Scholars ; National Science Fund of China for Distinguished Young Scholars |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/173263]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Guo, Zhimou; Liang, Xinmiao; Ye, Mingliang |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Dalian Med Univ, Affiliated Hosp 2, Dalian 116027, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qin, Hongqiang,Dong, Xuefang,Mao, Jiawei,et al. Highly Efficient Analysis of Glycoprotein Sialylation in Human Serum by Simultaneous Quantification of Glycosites and Site-Specific Glycoforms[J]. JOURNAL OF PROTEOME RESEARCH,2019,18(9):3439-3446. |
| APA | Qin, Hongqiang.,Dong, Xuefang.,Mao, Jiawei.,Chen, Yao.,Dong, Mingming.,...&Ye, Mingliang.(2019).Highly Efficient Analysis of Glycoprotein Sialylation in Human Serum by Simultaneous Quantification of Glycosites and Site-Specific Glycoforms.JOURNAL OF PROTEOME RESEARCH,18(9),3439-3446. |
| MLA | Qin, Hongqiang,et al."Highly Efficient Analysis of Glycoprotein Sialylation in Human Serum by Simultaneous Quantification of Glycosites and Site-Specific Glycoforms".JOURNAL OF PROTEOME RESEARCH 18.9(2019):3439-3446. |
入库方式： OAI收割
来源：大连化学物理研究所
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