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The efficient profiling of serum N-linked glycans by a highly porous 3D graphene composite

文献类型：期刊论文

作者Niu, Huan1,2; Li, Xin1,2; Peng, Jiaxi1,2; Zhang, Hongyan1,2; Zhao, Xingyun1,2; Zhou, Xiaoyu1,2; Yu, Dongping1,2; Liu, Xinyu1; Wu, Ren'an1
刊名ANALYST
出版日期2019-09-07
卷号144期号:17页码:5261-5270
ISSN号0003-2654
DOI10.1039/c9an01119f
通讯作者Wu, Ren'an(wurenan@dicp.ac.cn)
英文摘要In this work, an enrichment approach for the profiling of N-linked glycans was developed by utilizing a highly porous 3D graphene composite fabricated from graphene oxide nanosheets and a phenol-formaldehyde polymer via graphitization and KOH activation. In tailoring the large surface area (ca. 2213 m(2) g(-1)) and 3D-layered mesoporous structure, the 3D graphene composite demonstrated not only high efficiency in glycan enrichment but also the size-exclusion effect against residual protein interference. For a standard protein ovalbumin digest, 26 N-linked glycans were identified with good repeatability, and the detection limit was as low as 0.25 ng mu L-1 with the identification of 13 N-linked glycans (S/N > 10). When the mass ratio of the ovalbumin digest to the interfering proteins, i.e., bovine serum albumin and ovalbumin was 1 : 2000 : 2000, 18 N-linked glycans could still be detected with sufficient signal intensities. From a 60 nL minute complex human serum sample, up to 53 N-linked glycans with S/N > 10 were identified after the 3D graphene enrichment, while only 20 N-linked glycans were identified by the porous graphitized carbon material used for comparison. In addition, the application of the 3D graphene composite in profiling the up-regulated and down-regulated N-linked glycans from the real clinical serum samples of ovarian cancer patients confirmed the potential of the 3D graphene composite for analyzing minute and complicated biological samples.
WOS关键词SELECTIVE ENRICHMENT ; FACILE PREPARATION ; HIGH-SENSITIVITY ; HIGH-THROUGHPUT ; CARBON ; GLYCOSYLATION ; GLYCOMICS ; CANCER ; OLIGOSACCHARIDES ; GLYCOPROTEOMICS
资助项目National Natural Science Foundation of China[21675156] ; National Natural Science Foundation of China[21375125] ; Instrument Developing Project of the Chinese Academy of Sciences[YZ201503] ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics[DICP TMSR201601]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000483593000022
出版者ROYAL SOC CHEMISTRY
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics
源URL[http://cas-ir.dicp.ac.cn/handle/321008/173309]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者Wu, Ren'an
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Niu, Huan,Li, Xin,Peng, Jiaxi,et al. The efficient profiling of serum N-linked glycans by a highly porous 3D graphene composite[J]. ANALYST,2019,144(17):5261-5270.
APA Niu, Huan.,Li, Xin.,Peng, Jiaxi.,Zhang, Hongyan.,Zhao, Xingyun.,...&Wu, Ren'an.(2019).The efficient profiling of serum N-linked glycans by a highly porous 3D graphene composite.ANALYST,144(17),5261-5270.
MLA Niu, Huan,et al."The efficient profiling of serum N-linked glycans by a highly porous 3D graphene composite".ANALYST 144.17(2019):5261-5270.

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来源：大连化学物理研究所

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