Donkey milk oligosaccharides influence the growth-related characteristics of intestinal cells and induce G2/M growth arrest via the p38 pathway in HT-29 cells
文献类型：期刊论文
| 作者 | Wang, Jingxuan1; Lei, Beibei1; Yan, Jingyu2; Li, Jiaqi2; Zhou, Xiangshan4; Ren, Fazheng1,3; Guo, Huiyuan1 |
| 刊名 | FOOD & FUNCTION
 |
| 出版日期 | 2019-08-01 |
| 卷号 | 10期号:8页码:4823-4833 |
| ISSN号 | 2042-6496 |
| DOI | 10.1039/c8fo02584c |
| 通讯作者 | Guo, Huiyuan(guohuiyuan@cau.edu.ar) |
| 英文摘要 | Donkey milk is considered to be a valuable nutritional source. Deeper knowledge of the constituents of donkey milk is necessary. As multifunctional components of milk, oligosaccharides have been reported to have the potential to support intestine development. We studied the composition and content of donkey milk oligosaccharides (DMOs). Sialylated oligosaccharides were found to be the primary oligosaccharides in DMOs, consisting of 3 '-sialyllactose (SL) and 6 '-SL. The amount of 3 '-SL and 6 '-SL in donkey milk was 18.3 +/- 0.7 mg L-1 and 33.1 +/- 0.7 mg L-1, respectively. Moreover, we found that DMOs induced differentiation, promoted apoptosis and inhibited proliferation in HT-29, Caco-2 and HIEC cells in a concentration-dependent manner, suggesting that DMOs promote maturation of intestinal epithelial cells. The mechanism of the DMOs' effects on HT-29 cells was associated with activation of the p38 pathway and cell cycle arrest at the G2/M phase. Our research will help understand the biological functions of DMOs and assess their potential roles in infant nutrition. |
| WOS关键词 | CYCLE ARREST ; SIALYLATED OLIGOSACCHARIDES ; ACTIVATION ; MATURATION ; BARRIER ; KINASE ; PHASE ; MODEL ; GUT |
| 资助项目 | Beijing Science and Technology Project[Z181100009318005] ; 111 project from the Education Ministry of China[B18053] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Food Science & Technology |
| 语种 | 英语 |
| 出版者 | ROYAL SOC CHEMISTRY |
| WOS记录号 | WOS:000481481700028 |
| 资助机构 | Beijing Science and Technology Project ; Beijing Science and Technology Project ; 111 project from the Education Ministry of China ; 111 project from the Education Ministry of China ; Beijing Science and Technology Project ; Beijing Science and Technology Project ; 111 project from the Education Ministry of China ; 111 project from the Education Ministry of China ; Beijing Science and Technology Project ; Beijing Science and Technology Project ; 111 project from the Education Ministry of China ; 111 project from the Education Ministry of China ; Beijing Science and Technology Project ; Beijing Science and Technology Project ; 111 project from the Education Ministry of China ; 111 project from the Education Ministry of China |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/173503]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Guo, Huiyuan |
| 作者单位 | 1.China Agr Univ, Coll Food Sci & Nutr Engn, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Beijing 100083, Peoples R China 2.Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 3.China Agr Univ, Minist Educ & Beijing Govt, Key Lab Funct Dairy, Beijing 100083, Peoples R China 4.Dong E E Jiao Co Ltd, Natl Engn Res Ctr Gelatin Based Ttadit Chinese Me, 78 E Jiao St, Done E Country 252201, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Jingxuan,Lei, Beibei,Yan, Jingyu,et al. Donkey milk oligosaccharides influence the growth-related characteristics of intestinal cells and induce G2/M growth arrest via the p38 pathway in HT-29 cells[J]. FOOD & FUNCTION,2019,10(8):4823-4833. |
| APA | Wang, Jingxuan.,Lei, Beibei.,Yan, Jingyu.,Li, Jiaqi.,Zhou, Xiangshan.,...&Guo, Huiyuan.(2019).Donkey milk oligosaccharides influence the growth-related characteristics of intestinal cells and induce G2/M growth arrest via the p38 pathway in HT-29 cells.FOOD & FUNCTION,10(8),4823-4833. |
| MLA | Wang, Jingxuan,et al."Donkey milk oligosaccharides influence the growth-related characteristics of intestinal cells and induce G2/M growth arrest via the p38 pathway in HT-29 cells".FOOD & FUNCTION 10.8(2019):4823-4833. |
入库方式： OAI收割
来源：大连化学物理研究所
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