中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Smart polymers driven by multiple and tunable hydrogen bonds for intact phosphoprotein enrichment

文献类型:期刊论文

作者Zhang, Xiaofei1; Lu, Qi2,3; Chen, Cheng1; Li, Xiuling1; Qing, Guangyan1; Sun, Taolei2; Liang, Xinmiao1
刊名SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS
出版日期2019-12-31
卷号20期号:1页码:858-869
ISSN号1468-6996
关键词Phosphoprotein enrichment hydrogen bond
DOI10.1080/14686996.2019.1643259
通讯作者Li, Xiuling(lixiuling@dicp.ac.cn) ; Qing, Guangyan(qinggy@dicp.ac.cn)
英文摘要Separation of phosphoproteins is essential for understanding their vital roles in biological processes and pathology. Transition metal-based receptors and antibodies, the routinely used materials for phosphoproteins enrichment, both suffer from low sensitivity, low recovery and coverage. In this work, a novel smart copolymer material was synthesized by modifying porous silica gel with a poly[(N-isopropylacrylamide-co-4-(3-acryloylthioureido) benzoic acid)0.35] (denoted as NIPAAm-co-ATBA0.35@SiO2). Driven by the hydrogen bonds complexation of ATBA monomers with phosphate groups, the copolymer-modified surface exhibited a remarkable adsorption toward native alpha-casein (a model phosphoprotein), accompanied with signi?cant changes in surface viscoelasticity and roughness. Moreover, this adsorption was tunable and critically dependent on the polarity of carrier solvent. Bene?ting from these features, selective enrichment of phosphoprotein was obtained using NIPAAm-co-ATBA(0.35)@SiO2 under a dispersive solid-phase extraction (dSPE) mode. This result displays a good potential of smart polymeric materials in phosphoprotein enrichment, which may facilitate top-down phosphoproteomics studies.
WOS关键词SELECTIVE ENRICHMENT ; PROTEIN ; PHOSPHORYLATION ; SEPARATION ; MODEL
资助项目National Natural Science Foundation of China[21775148] ; National Natural Science Foundation of China[51533007] ; National Natural Science Foundation of China[21775116] ; National Natural Science Foundation of China[51473131] ; National Natural Science Foundation of China[51521001] ; DICP Innovation Funding[DICP-RC201801]
WOS研究方向Materials Science
语种英语
出版者TAYLOR & FRANCIS LTD
WOS记录号WOS:000482379800001
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; DICP Innovation Funding ; DICP Innovation Funding ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; DICP Innovation Funding ; DICP Innovation Funding ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; DICP Innovation Funding ; DICP Innovation Funding ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; DICP Innovation Funding ; DICP Innovation Funding
源URL[http://cas-ir.dicp.ac.cn/handle/321008/173561]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者Li, Xiuling; Qing, Guangyan
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Peoples R China
2.Wuhan Univ Technol, State Key Lab Adv Technol Mat Synth & Proc, Wuhan, Hubei, Peoples R China
3.Jushi Grp Co, Res & Dev Ctr, Tongxiang City, Zhejiang, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Xiaofei,Lu, Qi,Chen, Cheng,et al. Smart polymers driven by multiple and tunable hydrogen bonds for intact phosphoprotein enrichment[J]. SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS,2019,20(1):858-869.
APA Zhang, Xiaofei.,Lu, Qi.,Chen, Cheng.,Li, Xiuling.,Qing, Guangyan.,...&Liang, Xinmiao.(2019).Smart polymers driven by multiple and tunable hydrogen bonds for intact phosphoprotein enrichment.SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS,20(1),858-869.
MLA Zhang, Xiaofei,et al."Smart polymers driven by multiple and tunable hydrogen bonds for intact phosphoprotein enrichment".SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS 20.1(2019):858-869.

入库方式: OAI收割

来源:大连化学物理研究所

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