Smart polymers driven by multiple and tunable hydrogen bonds for intact phosphoprotein enrichment
文献类型:期刊论文
作者 | Zhang, Xiaofei1; Lu, Qi2,3; Chen, Cheng1; Li, Xiuling1; Qing, Guangyan1; Sun, Taolei2; Liang, Xinmiao1 |
刊名 | SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS |
出版日期 | 2019-12-31 |
卷号 | 20期号:1页码:858-869 |
ISSN号 | 1468-6996 |
关键词 | Phosphoprotein enrichment hydrogen bond |
DOI | 10.1080/14686996.2019.1643259 |
通讯作者 | Li, Xiuling(lixiuling@dicp.ac.cn) ; Qing, Guangyan(qinggy@dicp.ac.cn) |
英文摘要 | Separation of phosphoproteins is essential for understanding their vital roles in biological processes and pathology. Transition metal-based receptors and antibodies, the routinely used materials for phosphoproteins enrichment, both suffer from low sensitivity, low recovery and coverage. In this work, a novel smart copolymer material was synthesized by modifying porous silica gel with a poly[(N-isopropylacrylamide-co-4-(3-acryloylthioureido) benzoic acid)0.35] (denoted as NIPAAm-co-ATBA0.35@SiO2). Driven by the hydrogen bonds complexation of ATBA monomers with phosphate groups, the copolymer-modified surface exhibited a remarkable adsorption toward native alpha-casein (a model phosphoprotein), accompanied with signi?cant changes in surface viscoelasticity and roughness. Moreover, this adsorption was tunable and critically dependent on the polarity of carrier solvent. Bene?ting from these features, selective enrichment of phosphoprotein was obtained using NIPAAm-co-ATBA(0.35)@SiO2 under a dispersive solid-phase extraction (dSPE) mode. This result displays a good potential of smart polymeric materials in phosphoprotein enrichment, which may facilitate top-down phosphoproteomics studies. |
WOS关键词 | SELECTIVE ENRICHMENT ; PROTEIN ; PHOSPHORYLATION ; SEPARATION ; MODEL |
资助项目 | National Natural Science Foundation of China[21775148] ; National Natural Science Foundation of China[51533007] ; National Natural Science Foundation of China[21775116] ; National Natural Science Foundation of China[51473131] ; National Natural Science Foundation of China[51521001] ; DICP Innovation Funding[DICP-RC201801] |
WOS研究方向 | Materials Science |
语种 | 英语 |
出版者 | TAYLOR & FRANCIS LTD |
WOS记录号 | WOS:000482379800001 |
资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; DICP Innovation Funding ; DICP Innovation Funding ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; DICP Innovation Funding ; DICP Innovation Funding ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; DICP Innovation Funding ; DICP Innovation Funding ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; DICP Innovation Funding ; DICP Innovation Funding |
源URL | [http://cas-ir.dicp.ac.cn/handle/321008/173561] |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
通讯作者 | Li, Xiuling; Qing, Guangyan |
作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Peoples R China 2.Wuhan Univ Technol, State Key Lab Adv Technol Mat Synth & Proc, Wuhan, Hubei, Peoples R China 3.Jushi Grp Co, Res & Dev Ctr, Tongxiang City, Zhejiang, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, Xiaofei,Lu, Qi,Chen, Cheng,et al. Smart polymers driven by multiple and tunable hydrogen bonds for intact phosphoprotein enrichment[J]. SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS,2019,20(1):858-869. |
APA | Zhang, Xiaofei.,Lu, Qi.,Chen, Cheng.,Li, Xiuling.,Qing, Guangyan.,...&Liang, Xinmiao.(2019).Smart polymers driven by multiple and tunable hydrogen bonds for intact phosphoprotein enrichment.SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS,20(1),858-869. |
MLA | Zhang, Xiaofei,et al."Smart polymers driven by multiple and tunable hydrogen bonds for intact phosphoprotein enrichment".SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS 20.1(2019):858-869. |
入库方式: OAI收割
来源:大连化学物理研究所
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