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Advances in exosome isolation methods and their applications in proteomic analysis of biological samples

文献类型：期刊论文

作者Hou, Rui1; Li, Yilan2; Sui, Zhigang1; Yuan, Huiming1; Yang, Kaiguang1; Liang, Zhen1; Zhang, Lihua1; Zhang, Yukui1
刊名ANALYTICAL AND BIOANALYTICAL CHEMISTRY
出版日期2019-08-01
卷号411期号:21页码:5351-5361
ISSN号1618-2642
关键词Exosomes Extracellular vesicles Isolation Proteomic analysis
DOI10.1007/s00216-019-01982-0
通讯作者Yuan, Huiming(huimingyuan@dicp.ac.cn) ; Zhang, Lihua(lihuazhang@dicp.ac.cn)
英文摘要Exosomes are membrane-bound vesicles secreted by cells, and contain various important biological molecules, such as lipids, proteins, messenger RNAs, microRNAs, and noncoding RNAs. Emerging evidence demonstrates that proteomic analysis of exosomes is of great significance in studying metabolic diseases, tumor metastasis, immune regulation, and so forth. However, exosome proteomic analysis has high requirements with regard to the purity of collected exosomes. Here recent advances in the methods for isolating exosomes and their applications in proteomic analysis are summarized.
WOS关键词CELL-CULTURE SUPERNATANT ; EXTRACELLULAR VESICLES ; LIQUID-CHROMATOGRAPHY ; RAPID ISOLATION ; PLASMA ; BLOOD ; ULTRACENTRIFUGATION ; ENRICHMENT ; DELIVERY ; QUANTIFICATION
资助项目National Key R&D Program of China[2017YFA0505003] ; National Natural Science Foundation of China[21775149] ; National Natural Science Foundation of China[21725506] ; Innovation Program of Science and Research of Dalian Institute of Chemical Physics, Chinese Academy of Sciences[DICP TMSR201601]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
出版者SPRINGER HEIDELBERG
WOS记录号WOS:000479055200002
资助机构National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Innovation Program of Science and Research of Dalian Institute of Chemical Physics, Chinese Academy of Sciences ; Innovation Program of Science and Research of Dalian Institute of Chemical Physics, Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Innovation Program of Science and Research of Dalian Institute of Chemical Physics, Chinese Academy of Sciences ; Innovation Program of Science and Research of Dalian Institute of Chemical Physics, Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Innovation Program of Science and Research of Dalian Institute of Chemical Physics, Chinese Academy of Sciences ; Innovation Program of Science and Research of Dalian Institute of Chemical Physics, Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Innovation Program of Science and Research of Dalian Institute of Chemical Physics, Chinese Academy of Sciences ; Innovation Program of Science and Research of Dalian Institute of Chemical Physics, Chinese Academy of Sciences
源URL[http://cas-ir.dicp.ac.cn/handle/321008/173570]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者Yuan, Huiming; Zhang, Lihua
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Liaoning, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Hou, Rui,Li, Yilan,Sui, Zhigang,et al. Advances in exosome isolation methods and their applications in proteomic analysis of biological samples[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2019,411(21):5351-5361.
APA Hou, Rui.,Li, Yilan.,Sui, Zhigang.,Yuan, Huiming.,Yang, Kaiguang.,...&Zhang, Yukui.(2019).Advances in exosome isolation methods and their applications in proteomic analysis of biological samples.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,411(21),5351-5361.
MLA Hou, Rui,et al."Advances in exosome isolation methods and their applications in proteomic analysis of biological samples".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 411.21(2019):5351-5361.

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来源：大连化学物理研究所

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