A chemoenzymatic approach enables the site-specific conjugation of recombinant proteins
文献类型：期刊论文
| 作者 | Wang, Yan2,3; Dong, Mingming1,2; Ye, Mingliang2,3 |
| 刊名 | ELECTROPHORESIS
 |
| 出版日期 | 2019-08-01 |
| 卷号 | 40期号:16-17页码:2125-2128 |
| 关键词 | Biorthogonal chemistry Chemoenzymatic approach Site-specific conjugation Tyrosine phosphorylation |
| ISSN号 | 0173-0835 |
| DOI | 10.1002/elps.201800528 |
| 通讯作者 | Ye, Mingliang(mingliang@dicp.ac.cn) |
| 英文摘要 | Many biotechniques including protein microarray, drug screening, biosensors rely on the immobilization of recombinant proteins on the solid supports. It is well known that random orientation of the immobilized proteins could impair their biologic functions. Thus, it is very important to develop new site-specific immobilization approach. In this study, we presented a chemoenzymatic approach for site-specific conjugation of recombinant proteins onto solid support. In this strategy, the affinity tag on recombinant protein was enzymatically cleaved to expose the N-terminal serine, which was oxidized to carry an aldehyde group and was then covalently coupled to hydrazide resin through hydrazone ligation. As this approach takes advantage of the most frequently used TEV protease, it requires no further sequence design on recombinant protein. This method was validated by site specific coupling of a synthetic peptide and a recombinant protein onto solid supports. It was found that the site specific immobilized SH2 domain is functional and could be used to enrich tyrosine phosphorylated peptides. |
| WOS关键词 | IDENTIFICATION ; ENRICHMENT ; SORTASE ; QUANTIFICATION ; PEPTIDES |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000478750500013 |
| 出版者 | WILEY |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/173683]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Ye, Mingliang |
| 作者单位 | 1.Dalian Ocean Univ, Dalian, Peoples R China 2.Univ Chinese Acad Sci, CAS Key Lab Separat Sci Analyt Chem, Beijing 00049, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yan,Dong, Mingming,Ye, Mingliang. A chemoenzymatic approach enables the site-specific conjugation of recombinant proteins[J]. ELECTROPHORESIS,2019,40(16-17):2125-2128. |
| APA | Wang, Yan,Dong, Mingming,&Ye, Mingliang.(2019).A chemoenzymatic approach enables the site-specific conjugation of recombinant proteins.ELECTROPHORESIS,40(16-17),2125-2128. |
| MLA | Wang, Yan,et al."A chemoenzymatic approach enables the site-specific conjugation of recombinant proteins".ELECTROPHORESIS 40.16-17(2019):2125-2128. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。