Aptamer functionalized magnetic graphene oxide nanocomposites for highly selective capture of histones
文献类型:期刊论文
作者 | Chen, Yuanbo1,3; Jiang, Bo1; Hu, Yechen1,3; Deng, Nan1,2; Zhao, Baofeng1; Li, Xiao1; Liang, Zhen1; Zhang, Lihua1; Zhang, Yukui1 |
刊名 | ELECTROPHORESIS |
出版日期 | 2019-08-01 |
卷号 | 40期号:16-17页码:2135-2141 |
ISSN号 | 0173-0835 |
关键词 | Enrichment Graphene oxide Histones Magnetic nanoparticles |
DOI | 10.1002/elps.201900061 |
通讯作者 | Zhang, Lihua(lihuazhang@dicp.ac.cn) |
英文摘要 | The binding coverage of aptamer was an important restricted factor for aptamer-based affinity enrichment strategy for capturing target molecules. Herein, we designed and prepared aptamer functionalized graphene oxide based nanocomposites (GO/NH2-NTA/Fe3O4/PEI/Au), and the coverage density of aptamer was high to 33.1 nmol/mg. The high aptamer coverage density was contributed to the large surface area of graphene oxide. The successive modification of N alpha,N alpha-Bis(carboxymethyl)-L-lysine, magnetic nanoparticles, polyethylenimine, and Au nanoparticles ensured the histone purification with fast speed and high purity. Histones could be captured rapidly and specifically from nucleoproteins by our aptamer based purification strategy, while traditional acid-extraction could not specifically enrich histones. Compared with traditional acid-extraction method, rapid and efficient discovery of histones and their post-translational modifications, such as several kinds of methylation at H3.1K9 and H3.1K27, were achieved confidently. It demonstrated that our aptamer functionalized magnetic graphene oxide nanocomposites have a great potential for histone analysis. |
WOS关键词 | IN-VITRO SELECTION ; ENRICHMENT ; IMMOBILIZATION ; COMPOSITES ; EVOLUTION |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
语种 | 英语 |
出版者 | WILEY |
WOS记录号 | WOS:000478750500015 |
源URL | [http://cas-ir.dicp.ac.cn/handle/321008/173993] |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
通讯作者 | Zhang, Lihua |
作者单位 | 1.Chinese Acad Sci, Natl Chromatog Res & Anal Ctr, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Zhengzhou Tobacco Res Inst CNTC, Zhengzhou, Henan, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China |
推荐引用方式 GB/T 7714 | Chen, Yuanbo,Jiang, Bo,Hu, Yechen,et al. Aptamer functionalized magnetic graphene oxide nanocomposites for highly selective capture of histones[J]. ELECTROPHORESIS,2019,40(16-17):2135-2141. |
APA | Chen, Yuanbo.,Jiang, Bo.,Hu, Yechen.,Deng, Nan.,Zhao, Baofeng.,...&Zhang, Yukui.(2019).Aptamer functionalized magnetic graphene oxide nanocomposites for highly selective capture of histones.ELECTROPHORESIS,40(16-17),2135-2141. |
MLA | Chen, Yuanbo,et al."Aptamer functionalized magnetic graphene oxide nanocomposites for highly selective capture of histones".ELECTROPHORESIS 40.16-17(2019):2135-2141. |
入库方式: OAI收割
来源:大连化学物理研究所
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