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Analysis of oligonucleotides by ion-pair reversed-phase liquid chromatography coupled with positive mode electrospray ionization mass spectrometry

文献类型：期刊论文

作者Weng, Guofeng2,3; Sun, Binwen1,3; Liu, Zheyi1,3; Wang, Fangjun3; Pan, Yuanjiang2
刊名ANALYTICAL AND BIOANALYTICAL CHEMISTRY
出版日期2019-07-01
卷号411期号:18页码:4167-4173
关键词Oligonucleotide Ion-pair reversed-phase liquid chromatography Positive mode mass spectrometry In-source energy
ISSN号1618-2642
DOI10.1007/s00216-019-01819-w
通讯作者Wang, Fangjun(wangfj@dicp.ac.cn) ; Pan, Yuanjiang(panyuanjiang@zju.edu.cn)
英文摘要Oligonucleotides are usually analyzed by ion-pair reversed-phase liquid chromatography (IP-RPLC) coupled with negative mode electrospray ionization mass spectrometry (ESI-MS) due to their highly negative charged phosphodiester backbones. Herein, the signal suppression effect of triethylamine (TEA) adducts caused the ion-pair reagent TEA/hexafluoroisopropanol (HFIP) is greatly alleviated after improving the in-source energy in positive mode ESI-MS. This strategy is applied for different RNA sequencing through analyzing their formic acid hydrolysates via IP-RPLC MS. Comparing with negative ion mode, we demonstrate that IP-RPLC MS analysis in positive ion mode is more suitable for RNA sequencing with fewer contaminant interferences. Finally, simultaneous online separation and detection of oligonucleotides and protein digests are achieved in positive ion mode IP-RPLC MS analysis with little interference to each other.
WOS关键词CHEMICALLY-MODIFIED OLIGONUCLEOTIDES ; CROSS-LINKING ; POSTTRANSLATIONAL MODIFICATIONS ; PROTEOMIC ANALYSIS ; CATION-EXCHANGE ; PERFORMANCE ; RNA ; SEPARATION ; ACID ; SUPPRESSION
资助项目National Key R&D Program of China[2016YFF0200503] ; National Natural Science Foundation of China[91853101] ; National Natural Science Foundation of China[21675152] ; National Natural Science Foundation of China[21532005]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
WOS记录号WOS:000474266400021
出版者SPRINGER HEIDELBERG
资助机构National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
源URL[http://cas-ir.dicp.ac.cn/handle/321008/175162]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者Wang, Fangjun; Pan, Yuanjiang
作者单位1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Zhejiang Univ, Dept Chem, Hangzhou 310027, Zhejiang, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Liaoning, Peoples R China
推荐引用方式
GB/T 7714		 Weng, Guofeng,Sun, Binwen,Liu, Zheyi,et al. Analysis of oligonucleotides by ion-pair reversed-phase liquid chromatography coupled with positive mode electrospray ionization mass spectrometry[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2019,411(18):4167-4173.
APA Weng, Guofeng,Sun, Binwen,Liu, Zheyi,Wang, Fangjun,&Pan, Yuanjiang.(2019).Analysis of oligonucleotides by ion-pair reversed-phase liquid chromatography coupled with positive mode electrospray ionization mass spectrometry.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,411(18),4167-4173.
MLA Weng, Guofeng,et al."Analysis of oligonucleotides by ion-pair reversed-phase liquid chromatography coupled with positive mode electrospray ionization mass spectrometry".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 411.18(2019):4167-4173.

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来源：大连化学物理研究所

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