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Integrated Microfluidic Device for Enrichment and Identification of Circulating Tumor Cells from the Blood of Patients with Colorectal Cancer

文献类型：期刊论文

作者Su, Wentao2,3; Yu, Hao2; Jiang, Lei2,3; Chen, Wenwen2,3; Li, Hongjing4; Qin, Jianhua1,2,3,5
刊名DISEASE MARKERS
出版日期2019
页码9
ISSN号0278-0240
DOI10.1155/2019/8945974
通讯作者Qin, Jianhua(jhqin@dicp.ac.cn)
英文摘要Integrated device with high purity for circulating tumor cell (CTC) identification has been regarded as a key goal to make CTC analysis a bench-to-bedside technology. Here, we have developed a novel integrated microfluidic device that can enrich and identify the CTCs from the blood of patients with colorectal cancer. To enrich CTCs from whole blood, microfabricated trapping chambers were included in the miniaturized device, allowing for the isolation of tumor cells based on differences in size and deformability between tumor and normal blood cells. Microvalves were also introduced sequentially in the device, enabling automatic CTC enrichment as well as immunostaining reagent delivery. Under optimized conditions, the whole blood spiked with caco-2 cells passing through the microfluidic device after leukocyte depletion and approximately 73% of caco-2 cells were identified by epithelial cell adhesion molecule (EpCAM) staining. In clinical samples, CTCs were detectable from all patients with advanced colorectal cancer within 3 h. In contrast, the number of CTCs captured on the device from the blood of healthy donors was significantly lower than that from the patients, suggesting the utilization of the integrated device for further molecular analyses of CTCs.
WOS关键词MICROCAVITY ARRAY ; SEPARATION ; SIZE
资助项目National Key R&D Program of China[2017YFB0405404] ; National Nature Science Foundation of China[21607151] ; National Nature Science Foundation of China[81573394] ; National Nature Science Foundation of China[31671038] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA16020900] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB29050301] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB32030200] ; National Science and Technology Major Project[2018ZX09201017-001-001] ; Innovation Program of Science and Research from the DICP, CAS[DICP TMSR201601]
WOS研究方向Biotechnology & Applied Microbiology ; Genetics & Heredity ; Research & Experimental Medicine ; Pathology
语种英语
出版者HINDAWI LTD
WOS记录号WOS:000475590900001
资助机构National Key R&D Program of China ; National Key R&D Program of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Science and Technology Major Project ; National Science and Technology Major Project ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS ; National Key R&D Program of China ; National Key R&D Program of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Science and Technology Major Project ; National Science and Technology Major Project ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS ; National Key R&D Program of China ; National Key R&D Program of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Science and Technology Major Project ; National Science and Technology Major Project ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS ; National Key R&D Program of China ; National Key R&D Program of China ; National Nature Science Foundation of China ; National Nature Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Science and Technology Major Project ; National Science and Technology Major Project ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS
源URL[http://cas-ir.dicp.ac.cn/handle/321008/175214]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者Qin, Jianhua
作者单位1.Chinese Acad Sci, Inst Stem Cell & Regenerat, Beijing, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Dalian Med Univ, Affiliated Hosp 1, Dalian, Peoples R China
5.Chinese Acad Sci, CAS Ctr Excellence Brain Sci & Intelligence Techn, Shanghai, Peoples R China
推荐引用方式
GB/T 7714		 Su, Wentao,Yu, Hao,Jiang, Lei,et al. Integrated Microfluidic Device for Enrichment and Identification of Circulating Tumor Cells from the Blood of Patients with Colorectal Cancer[J]. DISEASE MARKERS,2019:9.
APA Su, Wentao,Yu, Hao,Jiang, Lei,Chen, Wenwen,Li, Hongjing,&Qin, Jianhua.(2019).Integrated Microfluidic Device for Enrichment and Identification of Circulating Tumor Cells from the Blood of Patients with Colorectal Cancer.DISEASE MARKERS,9.
MLA Su, Wentao,et al."Integrated Microfluidic Device for Enrichment and Identification of Circulating Tumor Cells from the Blood of Patients with Colorectal Cancer".DISEASE MARKERS (2019):9.

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来源：大连化学物理研究所

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