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Preparation of glutathione-functionalized zwitterionic silica material for efficient enrichment of sialylated N-glycopeptides

文献类型：期刊论文

作者Fu, Dongmei1,2; Liu, Yujie2,3; Shen, Aijin2; Xiao, Yuansheng2; Yu, Long2; Liang, Xinmiao2
刊名ANALYTICAL AND BIOANALYTICAL CHEMISTRY
出版日期2019-07-01
卷号411期号:18页码:4131-4140
ISSN号1618-2642
关键词Glycopeptide enrichment Divinyl sulfone activation Hydrophilic interaction chromatography Mass spectrometry
DOI10.1007/s00216-019-01661-0
通讯作者Yu, Long(yulong@dicp.ac.cn) ; Liang, Xinmiao(liangxm@dicp.ac.cn)
英文摘要A glutathione (GSH)-functionalized silica material was prepared using divinyl sulfone activation chemistry (named SiO2-DVS-GSH). The successful synthesis of the SiO2-DVS-GSH material was confirmed by FT-IR, elemental analysis, and zeta potential analysis. The effects of water content, pH value, and salt concentration in the mobile phase on the model compound (uracil, uridine, cytosine, cytidine, guanosine, xanthosine, orotic acid) retention was studied, and a hydrophilic interaction liquid chromatography (HILIC) retention feature together with electrostatic interaction of the SiO2-DVS-GSH material was observed. The prepared stationary phase was further applied for the separation of oligosaccharide. In addition, the SiO2-DVS-GSH material displayed remarkable selectivity and specificity for the sialylated N-glycopeptides' enrichment from bovine fetuin tryptic digests, even at a mass ratio of 1:1000 (w/w) to bovine serum albumin (BSA, non-glycosylated protein), showing superior performance compared to commercial ZIC-HILIC material. Moreover, the SiO2-DVS-GSH material behaved well in the N-glycopeptides' enrichment from human serum, demonstrating its promising potential for glycoproteomics of complex biological samples.
WOS关键词GLYCANS ; SULFONE ; SEPARATION ; AFFINITY ; BIOMARKERS ; ENZYMES ; PHASE
资助项目National Natural Science Foundation of China[21775148] ; Natural Science Foundation of Liaoning Province[20180550134]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
出版者SPRINGER HEIDELBERG
WOS记录号WOS:000474266400017
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Liaoning Province ; Natural Science Foundation of Liaoning Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Liaoning Province ; Natural Science Foundation of Liaoning Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Liaoning Province ; Natural Science Foundation of Liaoning Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Liaoning Province ; Natural Science Foundation of Liaoning Province
源URL[http://cas-ir.dicp.ac.cn/handle/321008/175373]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者Yu, Long; Liang, Xinmiao
作者单位1.Dalian Polytech Univ, Sch Biol Engn, Dalian 116034, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.Guangdong Univ Technol, Inst Biomed & Pharmaceut Sci, Guangzhou 510006, Guangdong, Peoples R China
推荐引用方式
GB/T 7714		 Fu, Dongmei,Liu, Yujie,Shen, Aijin,et al. Preparation of glutathione-functionalized zwitterionic silica material for efficient enrichment of sialylated N-glycopeptides[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2019,411(18):4131-4140.
APA Fu, Dongmei,Liu, Yujie,Shen, Aijin,Xiao, Yuansheng,Yu, Long,&Liang, Xinmiao.(2019).Preparation of glutathione-functionalized zwitterionic silica material for efficient enrichment of sialylated N-glycopeptides.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,411(18),4131-4140.
MLA Fu, Dongmei,et al."Preparation of glutathione-functionalized zwitterionic silica material for efficient enrichment of sialylated N-glycopeptides".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 411.18(2019):4131-4140.

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来源：大连化学物理研究所

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