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Cleavable hydrophobic derivatization strategy for enrichment and identification of phosphorylated lysine peptides

文献类型：期刊论文

作者Hu, Yechen1,2; Li, Yang1,2; Gao, Hang1,2; Jiang, Bo1; Zhang, Xiaodan1; Li, Xiao1; Wu, Qiong1,2; Liang, Zhen1; Zhang, Lihua1; Zhang, Yukui1
刊名ANALYTICAL AND BIOANALYTICAL CHEMISTRY
出版日期2019-07-01
卷号411期号:18页码:4159-4166
关键词Phosphorylated lysine peptides Cleavable hydrophobic derivatization N-Phosphorylation Proteomics Liquid chromatography-tandem mass spectrometry
ISSN号1618-2642
DOI10.1007/s00216-019-01770-w
通讯作者Zhang, Lihua(lihuazhang@dicp.ac.cn)
英文摘要Because of structural flexibility and acid lability, the identification of phosphorylated lysine (pLys) peptides is a great challenge. We report here a cleavable hydrophobic derivatization (CHD) strategy for the enrichment and identification of pLys peptides. First, 2,5-dioxopyrrolidin-1-yl-3-(decyldisulfanyl)propanoate was synthesized to react with dephosphorylated lysine peptides, and then the derived peptides were captured by a C-18 column, followed by cleavage of the hydrophobic chain, with the specific label left on the target peptides for further identification. By CHD, the enrichment of pLys peptides from interfering peptides (1:1000 mass ratio) was achieved. Furthermore, CHD was applied to screen the pLys targets from Escherichia coli lysates, and 39 pLys sites from 35 proteins were identified. Gene Ontology (GO) analysis showed that these proteins played vital roles in catabolism, metabolism, biogenesis, and biosynthetic processes. All these results demonstrate that CHD might pave the way for comprehensive profiling of the pLys proteome.
WOS关键词BOVINE LIVER ; 1-32P-PHOSPHOHISTIDINE 3-32P-PHOSPHOHISTIDINE ; PROTEIN ; 3-PHOSPHOHISTIDINE ; 6-PHOSPHOLYSINE
资助项目National Key Research and Development Program of China[2017YFA0505003] ; National Natural Science Foundation[91543201] ; National Natural Science Foundation[21505133] ; National Natural Science Foundation[21725506] ; Chinese Academy of Sciences Key Project in Frontier Science[QYZDY-SSW-SLH017] ; Innovation Program of Dalian Institute of Chemical Physics, Chinese Academy of Sciences Key[DICP TMSR201601]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
WOS记录号WOS:000474266400020
出版者SPRINGER HEIDELBERG
资助机构National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences Key Project in Frontier Science ; Chinese Academy of Sciences Key Project in Frontier Science ; Innovation Program of Dalian Institute of Chemical Physics, Chinese Academy of Sciences Key ; Innovation Program of Dalian Institute of Chemical Physics, Chinese Academy of Sciences Key ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences Key Project in Frontier Science ; Chinese Academy of Sciences Key Project in Frontier Science ; Innovation Program of Dalian Institute of Chemical Physics, Chinese Academy of Sciences Key ; Innovation Program of Dalian Institute of Chemical Physics, Chinese Academy of Sciences Key ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences Key Project in Frontier Science ; Chinese Academy of Sciences Key Project in Frontier Science ; Innovation Program of Dalian Institute of Chemical Physics, Chinese Academy of Sciences Key ; Innovation Program of Dalian Institute of Chemical Physics, Chinese Academy of Sciences Key ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences Key Project in Frontier Science ; Chinese Academy of Sciences Key Project in Frontier Science ; Innovation Program of Dalian Institute of Chemical Physics, Chinese Academy of Sciences Key ; Innovation Program of Dalian Institute of Chemical Physics, Chinese Academy of Sciences Key
源URL[http://cas-ir.dicp.ac.cn/handle/321008/175434]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者Zhang, Lihua
作者单位1.Chinese Acad Sci, CAS Key Lab Separat Sci Analyt Chem, Natl Chromatog R&D Ctr, Dalian Inst Chem Phys, 457 Zhongshan Rd, Dalian 116023, Liaoning, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Hu, Yechen,Li, Yang,Gao, Hang,et al. Cleavable hydrophobic derivatization strategy for enrichment and identification of phosphorylated lysine peptides[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2019,411(18):4159-4166.
APA Hu, Yechen.,Li, Yang.,Gao, Hang.,Jiang, Bo.,Zhang, Xiaodan.,...&Zhang, Yukui.(2019).Cleavable hydrophobic derivatization strategy for enrichment and identification of phosphorylated lysine peptides.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,411(18),4159-4166.
MLA Hu, Yechen,et al."Cleavable hydrophobic derivatization strategy for enrichment and identification of phosphorylated lysine peptides".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 411.18(2019):4159-4166.

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