RNA interference in the oleaginous yeast Rhodosporidium toruloides
文献类型：期刊论文
| 作者 | Liu, Xiangjian1,2; Zhang, Yue2,3; Liu, Hongdi2; Jiao, Xiang2; Zhang, Qi2; Zhang, Sufang2; Zhao, Zongbao Kent2 |
| 刊名 | FEMS YEAST RESEARCH
 |
| 出版日期 | 2019-05-01 |
| 卷号 | 19期号:3页码:10 |
| 关键词 | Rhodosporidium toruloides RNAi endoribonuclease Dicer ATG8 FAS1 FAS2 |
| ISSN号 | 1567-1356 |
| DOI | 10.1093/femsyr/foz031 |
| 通讯作者 | Zhang, Sufang(zsfjxy@dicp.ac.cn) |
| 英文摘要 | The red yeast Rhodosporidium toruloides is an excellent microbial host for production of carotenoids, neutral lipids and valuable enzymes. In recent years, genetic tools for gene expression and gene disruption have been developed for this red yeast. However, methods remain limited in terms of fine-tuning gene expression. In this study, we first demonstrated successful implementation of RNA interference (RNAi) in R. toruloides NP11, which was applied to down-regulate the expression of autophagy related gene 8 (ATG8), and fatty acid synthase genes (FAS1 and FAS2), respectively. Compared with the control strain, RNAi-engineered strains showed a silencing efficiency ranging from 11% to 92%. The RNAi approach described here ensures selective inhibition of the target gene expression, and should expand our capacity in the genetic manipulation of R. toruloides for both fundamental research and advanced cell factory development. |
| WOS关键词 | DOUBLE-STRANDED-RNA ; GENE-EXPRESSION ; TRANSFORMATION ; RHODOTORULA ; SYSTEM ; GENOME ; CAROTENOIDS ; HYDROLYSATE ; REPRESSION ; PROMOTERS |
| 资助项目 | National Natural Science Foundation of China[51761145014] ; Dalian Institute of Chemical Physics, CAS[DICPQIBEBT UN201706] ; Dalian Institute of Chemical Physics, CAS[DMTO201701] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Microbiology ; Mycology |
| 语种 | 英语 |
| WOS记录号 | WOS:000474809700019 |
| 出版者 | OXFORD UNIV PRESS |
| 资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; Dalian Institute of Chemical Physics, CAS ; Dalian Institute of Chemical Physics, CAS ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Dalian Institute of Chemical Physics, CAS ; Dalian Institute of Chemical Physics, CAS ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Dalian Institute of Chemical Physics, CAS ; Dalian Institute of Chemical Physics, CAS ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Dalian Institute of Chemical Physics, CAS ; Dalian Institute of Chemical Physics, CAS |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/175443]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Zhang, Sufang |
| 作者单位 | 1.Dalian Polytech Univ, Sch Biol Engn, Dalian 116034, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biotechnol, 457 Zhongshan Rd, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Xiangjian,Zhang, Yue,Liu, Hongdi,et al. RNA interference in the oleaginous yeast Rhodosporidium toruloides[J]. FEMS YEAST RESEARCH,2019,19(3):10. |
| APA | Liu, Xiangjian.,Zhang, Yue.,Liu, Hongdi.,Jiao, Xiang.,Zhang, Qi.,...&Zhao, Zongbao Kent.(2019).RNA interference in the oleaginous yeast Rhodosporidium toruloides.FEMS YEAST RESEARCH,19(3),10. |
| MLA | Liu, Xiangjian,et al."RNA interference in the oleaginous yeast Rhodosporidium toruloides".FEMS YEAST RESEARCH 19.3(2019):10. |
入库方式： OAI收割
来源：大连化学物理研究所
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