中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 大连化学物理研究所
  3. 中国科学院大连化学物理研究所
Profiling of Human Milk Oligosaccharides for Lewis Epitopes and Secretor Status by Electrostatic Repulsion Hydrophilic Interaction Chromatography Coupled with Negative-Ion Electrospray Tandem Mass Spectrometry

文献类型：期刊论文

作者Yan, Jingyu1; Ding, Junjie1,2; Jin, Gaowa1; Duan, Zhaojun3; Yang, Fan1; Li, Dandi3; Zhou, Han1; Li, Ming4; Guo, Zhimou1; Chai, Wengang5
刊名ANALYTICAL CHEMISTRY
出版日期2019-07-02
卷号91期号:13页码:8199-8206
ISSN号0003-2700
DOI10.1021/acs.analchem.9b00687
通讯作者Guo, Zhimou(guozhimou@dicp.ac.cn) ; Chai, Wengang(w.chai@imperial.ac.uk) ; Liang, Xinmiao(liangxm@dicp.ac.cn)
英文摘要Human milk oligosaccharides (HMOs) are one of the most abundant ingredients in breast milk, and they play a beneficial role for newborns and are important for infant health. The peripheral fucosylated sequences of HMOs, such as the histo-blood group ABH(0) and Lewis a, b, x, and y antigens, are determined by the expression of the secretor (Se) and Lewis (Le) genes in the mammary gland, and are often the recognition motifs and serve as decoy receptors for microbes. In this work, we developed a method for determination of secretor status and Lewis blood phenotype and assignment of Lewis blood-group epitopes. The method was based on electrostatic repulsion/hydrophilic interaction chromatography coupled with tandem mass spectrometry (ERLIC-MS/MS). A specifically designed stationary phase, aspartic acid-bonded silica (ABS), was used to separate the acidic and neutral HMOs by electrostatic repulsion followed by HILIC. Negative-ion electrospray MS/MS was then used for analysis of secretor status and Lewis blood phenotypes and assignment of important epitopes of HMOs from the lactating mothers by selecting a specific set of unique fragment ions.
WOS关键词SIALYLATED OLIGOSACCHARIDES ; STRUCTURAL-ANALYSIS ; SEPARATION ; MODE
资助项目National Key Research and Development Program of China[2017YFD0400600] ; National Key Research and Development Program of China[2016YFB0301502] ; National Natural Science Foundation of China[U1608255] ; Nutrition and Care of Maternal & Child Research Fund Project of Guangzhou Biostime Institute of Nutrition Care[2016BINCMCF1102] ; March of Dimes Prematurity Research Center[22-FY18-821]
WOS研究方向Chemistry
语种英语
出版者AMER CHEMICAL SOC
WOS记录号WOS:000474477900032
资助机构National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Nutrition and Care of Maternal & Child Research Fund Project of Guangzhou Biostime Institute of Nutrition Care ; Nutrition and Care of Maternal & Child Research Fund Project of Guangzhou Biostime Institute of Nutrition Care ; March of Dimes Prematurity Research Center ; March of Dimes Prematurity Research Center ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Nutrition and Care of Maternal & Child Research Fund Project of Guangzhou Biostime Institute of Nutrition Care ; Nutrition and Care of Maternal & Child Research Fund Project of Guangzhou Biostime Institute of Nutrition Care ; March of Dimes Prematurity Research Center ; March of Dimes Prematurity Research Center ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Nutrition and Care of Maternal & Child Research Fund Project of Guangzhou Biostime Institute of Nutrition Care ; Nutrition and Care of Maternal & Child Research Fund Project of Guangzhou Biostime Institute of Nutrition Care ; March of Dimes Prematurity Research Center ; March of Dimes Prematurity Research Center ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Nutrition and Care of Maternal & Child Research Fund Project of Guangzhou Biostime Institute of Nutrition Care ; Nutrition and Care of Maternal & Child Research Fund Project of Guangzhou Biostime Institute of Nutrition Care ; March of Dimes Prematurity Research Center ; March of Dimes Prematurity Research Center
源URL[http://cas-ir.dicp.ac.cn/handle/321008/175520]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者Guo, Zhimou; Chai, Wengang; Liang, Xinmiao
作者单位1.Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Natl Inst Viral Dis Control & Prevent, Beijing 102206, Peoples R China
4.Dalian Med Univ, Coll Basic Med Sci, Dalian, Peoples R China
5.Imperial Coll London, Fac Med, Glycosci Lab, Hammersmith Campus,Du Cane Rd, London W12 0NN, England
推荐引用方式
GB/T 7714		 Yan, Jingyu,Ding, Junjie,Jin, Gaowa,et al. Profiling of Human Milk Oligosaccharides for Lewis Epitopes and Secretor Status by Electrostatic Repulsion Hydrophilic Interaction Chromatography Coupled with Negative-Ion Electrospray Tandem Mass Spectrometry[J]. ANALYTICAL CHEMISTRY,2019,91(13):8199-8206.
APA Yan, Jingyu.,Ding, Junjie.,Jin, Gaowa.,Duan, Zhaojun.,Yang, Fan.,...&Liang, Xinmiao.(2019).Profiling of Human Milk Oligosaccharides for Lewis Epitopes and Secretor Status by Electrostatic Repulsion Hydrophilic Interaction Chromatography Coupled with Negative-Ion Electrospray Tandem Mass Spectrometry.ANALYTICAL CHEMISTRY,91(13),8199-8206.
MLA Yan, Jingyu,et al."Profiling of Human Milk Oligosaccharides for Lewis Epitopes and Secretor Status by Electrostatic Repulsion Hydrophilic Interaction Chromatography Coupled with Negative-Ion Electrospray Tandem Mass Spectrometry".ANALYTICAL CHEMISTRY 91.13(2019):8199-8206.

入库方式： OAI收割

来源：大连化学物理研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。