Expanding of Phospholipid:Diacylglycerol AcylTransferase (PDAT) from Saccharomyces cerevisiae as Multifunctional Biocatalyst with Broad Acyl Donor/Acceptor Selectivity
文献类型：期刊论文
| 作者 | Feng, Yanbin; Zhang, Yunxiu; Ding, Wei; Wu, Peichun; Cao, Xupeng; Xue, Song |
| 刊名 | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
 |
| 出版日期 | 2019-07-01 |
| 卷号 | 188期号:3页码:824-835 |
| 关键词 | Acyltransferase PDAT Acyl donor Biocatalyst Enzyme Acyl acceptor |
| ISSN号 | 0273-2289 |
| DOI | 10.1007/s12010-019-02954-x |
| 通讯作者 | Cao, Xupeng(c_x_p@dicp.ac.cn) ; Xue, Song(xuesong@dicp.ac.cn) |
| 英文摘要 | Triacylglycerols are considered one of the most promising feedstocks for biofuels. Phospholipid:diacylglycerol acyltransferase (PDAT), responsible for the last step of triacylglycerol synthesis in the acyl-CoA-independent pathway, has attracted much attention by catalyzing membrane lipid transformation. However, due to lack of biochemical and enzymatic studies, PDAT has not carried forward in biocatalyst application. Here, the PDAT from Saccharomyces cerevisiae was expressed in Pichia pastoris. The purified enzymes were studied using different acyl donors and acceptors by thin layer chromatography and gas chromatography. In addition of the preferred acyl donor of PE and PC, the results identified that ScPDAT was capable of using broad acyl donors such as PA, PS, PG, MGDG, DGDG, and acyl-CoA, and ScPDAT was more likely to use unsaturated acyl donors comparing 18:0/18:1 to 18:0/18:0 phospholipids. With regard to acyl acceptors, ScPDAT preferred 1,2 to 1,3-diacylglycerol (DAG), while 12:0/12:0 DAG was identified as the optimal acyl acceptor, followed by 18:1/18:1 and 18:1/16:0 DAG. Additionally, ScPDAT reveals esterification activity that can utilize methanol as acyl acceptor to generate fatty acid methyl esters. The results fully expand the enzymatic selectivity of ScPDAT and provide fundamental knowledge for synthesis of triacylglycerol-derived biofuels. |
| WOS关键词 | DIACYLGLYCEROL ACYLTRANSFERASE ; PHOSPHOLIPID/DIACYLGLYCEROL ACYLTRANSFERASE ; TRIACYLGLYCEROL ; ENZYME ; PLANT ; OIL ; DEGRADATION ; METABOLISM ; YEAST |
| 资助项目 | National Natural Science Foundation of China[21576253] ; National Natural Science Foundation of China[21877110] ; National Natural Science Foundation of China[31470432] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000471851400016 |
| 出版者 | SPRINGER |
| 资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/175940]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Cao, Xupeng; Xue, Song |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng, Yanbin,Zhang, Yunxiu,Ding, Wei,et al. Expanding of Phospholipid:Diacylglycerol AcylTransferase (PDAT) from Saccharomyces cerevisiae as Multifunctional Biocatalyst with Broad Acyl Donor/Acceptor Selectivity[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2019,188(3):824-835. |
| APA | Feng, Yanbin,Zhang, Yunxiu,Ding, Wei,Wu, Peichun,Cao, Xupeng,&Xue, Song.(2019).Expanding of Phospholipid:Diacylglycerol AcylTransferase (PDAT) from Saccharomyces cerevisiae as Multifunctional Biocatalyst with Broad Acyl Donor/Acceptor Selectivity.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,188(3),824-835. |
| MLA | Feng, Yanbin,et al."Expanding of Phospholipid:Diacylglycerol AcylTransferase (PDAT) from Saccharomyces cerevisiae as Multifunctional Biocatalyst with Broad Acyl Donor/Acceptor Selectivity".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 188.3(2019):824-835. |
入库方式： OAI收割
来源：大连化学物理研究所
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