Measurement of ultra-trace level of intact oxytocin in plasma using SALLE combined with nano-LC-MS
文献类型：期刊论文
| 作者 | Liu, Dan1; Han, Xiaoli1; Liu, Xinxin1; Cheng, Mengchun1; He, Meixi2; Rainer, Gregor3; Gao, Huiyuan2; Zhang, Xiaozhe1 |
| 刊名 | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
 |
| 出版日期 | 2019-09-05 |
| 卷号 | 173页码:62-67 |
| ISSN号 | 0731-7085 |
| 关键词 | Oxytocin SALLE Nano-LC-MS Plasma |
| DOI | 10.1016/j.jpba.2019.04.023 |
| 通讯作者 | Gao, Huiyuan(sypugaohy@163.com) ; Zhang, Xiaozhe(zhangxz@dicp.ac.cn) |
| 英文摘要 | Measurement of peptides such as oxytocin in plasma is a critical challenge in clinical research because of their extreme low concentrations as well as the tremendous interferencing substances co-presented in plasma. In this study, we developed an efficient salt-out assisted liquid-liquid extraction (SALLE) to treat plasma, and then analyzed the samples using nano-LC-MS to quantify intact oxytocin (OT) in human and rat plasmas. Our results showed that the use of SALLE (Isopropanol/K2HPO4 (4M)) allows efficient removal of various disrupters, including proteins, inorganic salts, and lipids, which helps avoid the risk of blocked capillary columns and matrix effects. Moreover, instant SALLE can reduce the possible binding between OT and proteins, thus allowing high repeatability of OT extraction from the original plasma. This combination of SALLE and nano-LC-MS method provided in the end a 1 pg/m L of detection limit. Comparative analysis showed that the concentration of OT in the plasma taken from 12 volunteers ranged from 3 to 214 pg/m L, about one order less than those in the plasma of rats. Compared to the previously reported LC-MS and immunoassay methods, the combination of SALLE and nano-LC-MS permits reliable measurement of intact OT even in human plasma. Our approach may be an alternative method for quantitative determination of other ultra-trace peptides in plasma, which would help the investigators understand the role of peptides in behaviours and diseases. (C) 2019 Published by Elsevier B.V. |
| WOS关键词 | ENZYME-IMMUNOASSAY ; BLOOD ; SERUM ; QUANTIFICATION ; EXTRACTION |
| 资助项目 | CAS 100Talent Project ; [NSF81573830/81774154/21475128] ; [MOST2015DFG42460] |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE BV |
| WOS记录号 | WOS:000472238700008 |
| 资助机构 | CAS 100Talent Project ; CAS 100Talent Project ; CAS 100Talent Project ; CAS 100Talent Project ; CAS 100Talent Project ; CAS 100Talent Project ; CAS 100Talent Project ; CAS 100Talent Project |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/175970]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Gao, Huiyuan; Zhang, Xiaozhe |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Peoples R China 2.Shenyang Pharmaceut Univ, Minist Educ, Key Lab Struct Based Drug Design & Discovery, Shenyang 110016, Liaoning, Peoples R China 3.Univ Fribourg, Dept Med, Chemin Musee 5, CH-1700 Fribourg, Switzerland |
| 推荐引用方式 GB/T 7714 | Liu, Dan,Han, Xiaoli,Liu, Xinxin,et al. Measurement of ultra-trace level of intact oxytocin in plasma using SALLE combined with nano-LC-MS[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2019,173:62-67. |
| APA | Liu, Dan.,Han, Xiaoli.,Liu, Xinxin.,Cheng, Mengchun.,He, Meixi.,...&Zhang, Xiaozhe.(2019).Measurement of ultra-trace level of intact oxytocin in plasma using SALLE combined with nano-LC-MS.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,173,62-67. |
| MLA | Liu, Dan,et al."Measurement of ultra-trace level of intact oxytocin in plasma using SALLE combined with nano-LC-MS".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 173(2019):62-67. |
入库方式： OAI收割
来源：大连化学物理研究所
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