positiveeffectsofisopropanolasacoprecipitantinglycerol3phosphateacyltransferasecrystallization
文献类型：期刊论文
| 作者 | ZHANG Yunxiu2; FENG Yanbin2; WANG Yayue2; LIU Yinghui2; CAO Xupeng2; XUE Song2 |
| 刊名 | 中国海洋大学学报英文版
 |
| 出版日期 | 2019-01-01 |
| 卷号 | 018期号:001页码:227 |
| ISSN号 | 1672-5182 |
| 英文摘要 | Glycerol-3-phosphate acyltransferase(GPAT)is considered as the rate-limiting enzyme of glycerolipid synthesis pathway and the core element in lysophosphatidic acid(LPA)synthesis.For understanding its catalytic mechanism,the structural biology study is expected,but is always hindered by obtaining crystals for X-ray diffraction analysis.In this study,a progressive strategy to optimize the crystal of microalgae plastidial GPAT was presented.After the expression and purification of GPAT,the crystals were screened by hanging-drop and only clusters were obtained.The crystals were optimized by adjusting temperature,pH,protein concentration,or precipitant,but little improvement.To improve the interaction between protein and precipitant,the isopropanol was applied as co-precipitant.The qualified crystals formed.It’s suggested that isopropanol is critical to affect protein crystallization by altering polyethylene glycol(PEG)-water-protein interaction when PEG serves as precipitant.The resulting crystal diffracted to a resolution of 2.75?and belonged to space group P1,with unit-cell parameters a=50.79,b=80.09,c=88.21?,andα=62.85,β=73.04,γ=80.53°.This work introduced a new strategy to optimize the crystal of the heterogeneous catalysis enzymes like GPAT and provided the fundamental structural information for the oriented synthesis of marine microalgae glycerolipid. |
| 资助项目 | [National Natural Science Foundation of China] |
| 语种 | 英语 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/176357]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.中国科学院大学 2.Marine Bioengineering Group,Dalian Institute of Chemical Physics,Chinese Academy of Sciences |
| 推荐引用方式 GB/T 7714 | ZHANG Yunxiu,FENG Yanbin,WANG Yayue,et al. positiveeffectsofisopropanolasacoprecipitantinglycerol3phosphateacyltransferasecrystallization[J]. 中国海洋大学学报英文版,2019,018(001):227. |
| APA | ZHANG Yunxiu,FENG Yanbin,WANG Yayue,LIU Yinghui,CAO Xupeng,&XUE Song.(2019).positiveeffectsofisopropanolasacoprecipitantinglycerol3phosphateacyltransferasecrystallization.中国海洋大学学报英文版,018(001),227. |
| MLA | ZHANG Yunxiu,et al."positiveeffectsofisopropanolasacoprecipitantinglycerol3phosphateacyltransferasecrystallization".中国海洋大学学报英文版 018.001(2019):227. |
入库方式： OAI收割
来源：大连化学物理研究所
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