isolationandpurificationofunstableiridoidglucosidesfromtraditionalchinesemedicinebypreparativehighperformanceliquidchromatographycoupledwithsolidphaseextraction
文献类型：期刊论文
| 作者 | LI Cunman2; XIAO Yuansheng1; XUE Xingya1; FENG Jiatao1; ZHANG Xiuli1; LIANG Xinmiao1 |
| 刊名 | chemicalresearchinchineseuniversities
 |
| 出版日期 | 2011-01-01 |
| 卷号 | 027期号:003页码:392 |
| ISSN号 | 1005-9040 |
| 英文摘要 | An efficient preparative method was successfully developed for isolation and purification of unstable components from medicinal plant extracts, using a combined method of preparative high performance liquid chro-matography（HPLC） and solid-phase extraction（SPE）. The aim of this study was to obtain an effective method with high preparative efficiency and importantly to avoid the transformation of unstable compounds. The preparative HPLC system was based on an LC/MS controlled four-channel autopurification system. The SPE method was performed with a C18 packing material to trap the target compounds and to remove the acidic additive derived from the mobile phase. Using this method, the unstable iridoid glucosides（IGs） as model compounds were successfully isolated and purified from the extract of Hedyotis diffusa Willd. Six IGs（including one new minor IG） and one nucleotide compound were simultaneously obtained, each with a purity of 91% as determined by HPLC. The structures of the isolated compounds were identified by UPLC/Q-TOF MS, UV, 1D and/or 2D NMR. It was demonstrated that the combination of preparative HPLC with SPE is a versatile tool for preparative purification of unstable compounds from complex natural products. |
| 语种 | 英语 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/176706]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.中国科学院大连化学物理研究所 2.Hebei Research Centre of Analysis and Testing,Hebei University of Science and Technology |
| 推荐引用方式 GB/T 7714 | LI Cunman,XIAO Yuansheng,XUE Xingya,et al. isolationandpurificationofunstableiridoidglucosidesfromtraditionalchinesemedicinebypreparativehighperformanceliquidchromatographycoupledwithsolidphaseextraction[J]. chemicalresearchinchineseuniversities,2011,027(003):392. |
| APA | LI Cunman,XIAO Yuansheng,XUE Xingya,FENG Jiatao,ZHANG Xiuli,&LIANG Xinmiao.(2011).isolationandpurificationofunstableiridoidglucosidesfromtraditionalchinesemedicinebypreparativehighperformanceliquidchromatographycoupledwithsolidphaseextraction.chemicalresearchinchineseuniversities,027(003),392. |
| MLA | LI Cunman,et al."isolationandpurificationofunstableiridoidglucosidesfromtraditionalchinesemedicinebypreparativehighperformanceliquidchromatographycoupledwithsolidphaseextraction".chemicalresearchinchineseuniversities 027.003(2011):392. |
入库方式： OAI收割
来源：大连化学物理研究所
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