chemicallybondedpolyacrylamideviathioleneclickchemistryasseparationmaterialsforhydrophilicinteractionliquidchromatography
文献类型：期刊论文
| 作者 | Yang D(杨铎)2; Yu DP(俞冬萍)1; Dong XF(董雪芳)1; Shen AJ(沈爱金)1; Jin GW(金高娃)1; Guo ZM(郭志谋)1; Yan JY(闫竞宇)1; Liu MY(刘名扬)2; Liang XM(梁鑫淼)1 |
| 刊名 | chinesejournalofanalyticalchemistry
 |
| 出版日期 | 2015-01-01 |
| 卷号 | 43期号:10页码:1439 |
| ISSN号 | 0253-3820 |
| 英文摘要 | Thiol-ene click chemistry was applied to immobilize polyacrylamide onto alkenyl-modified silica to prepare a novel stationary phase for hydrophilic interaction liquid chromatography. Polyacrylamide was synthesized by reversible addition-fragmentation chain transfer reaction. Alkenyl functionalities were introduced onto the silica by the reaction of silica with the reaction product of 3-isocyanate opropyl trimethoxy silane, allylamine and pyridine in toluene to obtain alkenyl-modified silica (EUS, E-thiol-ene, U-urea, S-silica). The alkenyl-modified silica was then reacted with polyacrylamide in methanol at 55 degrees C for 48 h in the presence of 2,2-azo diisobutyl amidine hydrochloride to prepare a novel stationary phase (TE-UPAM, TE-thiol-ene, U-urea, PAM-polyacrylamide), for hydrophilic interaction liquid chromatography. This new stationary phase "TE-UPAM" was characterized by element analysis and infrared spectra, and the results showed that, compared with EUS, the carbon content of TE-UPAM increased and the characteristic peaks of multiple amide existed at 1636 and 1570 cm(-1). The effects of water content, salt concentration and pH of the mobile phase on the retention time of polar compounds were investigated. The results showed that the retention time of polar compounds decreased with the increase of water content. In contrast with acidic polar compounds, the retention time of neutral and basic polar compounds increased with the increase of salt concentration, and the retention time of basic polar compounds decreased with the decrease of pH value in the range of 3.3-4.8. The novel stationary phase behaved better than traditional silica stationary phase in the separation of uridine and bases. In addition, good separation of oligosaccharides by the novel stationary phase was also achieved, indicating great potential in the separation of polar compounds. |
| 资助项目 | [National Nature Science Foundation of China] |
| 语种 | 英语 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/176720]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.中国科学院大连化学物理研究所 2.大连交通大学 |
| 推荐引用方式 GB/T 7714 | Yang D,Yu DP,Dong XF,et al. chemicallybondedpolyacrylamideviathioleneclickchemistryasseparationmaterialsforhydrophilicinteractionliquidchromatography[J]. chinesejournalofanalyticalchemistry,2015,43(10):1439. |
| APA | 杨铎.,俞冬萍.,董雪芳.,沈爱金.,金高娃.,...&梁鑫淼.(2015).chemicallybondedpolyacrylamideviathioleneclickchemistryasseparationmaterialsforhydrophilicinteractionliquidchromatography.chinesejournalofanalyticalchemistry,43(10),1439. |
| MLA | 杨铎,et al."chemicallybondedpolyacrylamideviathioleneclickchemistryasseparationmaterialsforhydrophilicinteractionliquidchromatography".chinesejournalofanalyticalchemistry 43.10(2015):1439. |
入库方式： OAI收割
来源：大连化学物理研究所
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