purificationoftheatgrp7rrmdomainfromarabidopsisthalianaanditspreliminarystructureandbindinganalysis
文献类型：期刊论文
| 作者 | Chi Xiujuan4; Qiao Xiaoya3; Liu Ying2; Liu Huili1; Chen Lei1; Wang Jihui4; Ai Xuanjun3 |
| 刊名 | 波谱学杂志
 |
| 出版日期 | 2019-01-01 |
| 卷号 | 36期号:1页码:1 |
| ISSN号 | 1000-4556 |
| 英文摘要 | The glycine-rich RNA-binding protein, AtGrp7, is a component of a negative feedback loop in the circadian clock regulation of Arabidopsis thaliana. In our initial purification trial of the tobacco etch virus (TEV)-cleaved AtGrp7 RNA recognition motif (RRM) domain with the regular protocol, mixed ultraviolet signals of the target proteins and contaminants were observed. A two-step denaturing-refolding protocol was then tested, trying to solve the problem of impurities. The structure of the AtGrp71-90 RRM domain was fully recovered by quick-dilution refolding, evidenced by the fingerprint 1H-15N HSQC spectrum and CS-Rosetta model structures. Isothermal titration calorimetry (ITC) and NMR titration experiments further confirmed that the RRM domain of AtGrp71-90 had proper functions with regards to RNA/DNA binding. |
| 语种 | 英语 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/176756]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.中国科学院物理研究所 2.Division of Virology & Immunology, National Center for AIDS/STD Control and Prevention 3.中国科学院大连化学物理研究所 4.大连工业大学 |
| 推荐引用方式 GB/T 7714 | Chi Xiujuan,Qiao Xiaoya,Liu Ying,et al. purificationoftheatgrp7rrmdomainfromarabidopsisthalianaanditspreliminarystructureandbindinganalysis[J]. 波谱学杂志,2019,36(1):1. |
| APA | Chi Xiujuan.,Qiao Xiaoya.,Liu Ying.,Liu Huili.,Chen Lei.,...&Ai Xuanjun.(2019).purificationoftheatgrp7rrmdomainfromarabidopsisthalianaanditspreliminarystructureandbindinganalysis.波谱学杂志,36(1),1. |
| MLA | Chi Xiujuan,et al."purificationoftheatgrp7rrmdomainfromarabidopsisthalianaanditspreliminarystructureandbindinganalysis".波谱学杂志 36.1(2019):1. |
入库方式： OAI收割
来源：大连化学物理研究所
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