screeningofmetaboliteregulatinglacticacidproductionofexiguobacteriumaurantiacumatcc49676bymetabolicfingerprintanalysis
文献类型：期刊论文
| 作者 | Lian Rongwei2; Tian Jing2; Gao Peng1; Wang Xiyue1; Fei Xu2; Wang Yi2; Xu Guowang1 |
| 刊名 | 化学学报
 |
| 出版日期 | 2012-01-01 |
| 卷号 | 70期号:24页码:2513 |
| ISSN号 | 0567-7351 |
| 英文摘要 | A strain of Exiguobacterium aurantiacum ATCC49676 was found to be of great potential to produce lactic acid(LA). In order to screen the metabolites that might regulate or affect the acid yield, a relative higher LA production condition was defined through full factorial experiment design. Fresh cultures harvested from the basic and the higher LA production conditions were subjected to metabolic fingerprint analysis by gas chromatography-mass spectrometry. Significance analysis indicated a distinct change of an intercellular metabolite-L-glutamic acid (Glu). When ATCC49676 was treated by Glu, LA yield declined with the increasing added Glu concentrations. Relative enzyme quantification confirmed that Glu decreased the intracellular lactate dehydrogenase content. This study proved the merit of metabolic fingerprint analysis in exploring the phenotype specific intracellular metabolite and its potential roles in improving industrial fermentation efficiencies. |
| 语种 | 英语 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/177441]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.中国科学院大连化学物理研究所 2.大连工业大学 |
| 推荐引用方式 GB/T 7714 | Lian Rongwei,Tian Jing,Gao Peng,et al. screeningofmetaboliteregulatinglacticacidproductionofexiguobacteriumaurantiacumatcc49676bymetabolicfingerprintanalysis[J]. 化学学报,2012,70(24):2513. |
| APA | Lian Rongwei.,Tian Jing.,Gao Peng.,Wang Xiyue.,Fei Xu.,...&Xu Guowang.(2012).screeningofmetaboliteregulatinglacticacidproductionofexiguobacteriumaurantiacumatcc49676bymetabolicfingerprintanalysis.化学学报,70(24),2513. |
| MLA | Lian Rongwei,et al."screeningofmetaboliteregulatinglacticacidproductionofexiguobacteriumaurantiacumatcc49676bymetabolicfingerprintanalysis".化学学报 70.24(2012):2513. |
入库方式： OAI收割
来源：大连化学物理研究所
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