13aminopropyl3butylimidazoliumbromideforcarboxylgroupderivatizationpotentialapplicationsinhighsensitivitypeptideidentificationbymassspectrometry
文献类型：期刊论文
| 作者 | Qiao Xiaoqiang; Zhou Yuan; Hou Chunyan; Zhang Xiaodan; Yang Kaiguang; Zhang Lihua; Zhang Yukui |
| 刊名 | sciencechinalifesciences
 |
| 出版日期 | 2013-01-01 |
| 卷号 | 56期号:3页码:240 |
| ISSN号 | 1674-7305 |
| 英文摘要 | The cationic reagent 1-(3-aminopropyl)-3-butylimidazolium bromide(BAPI) was exploited for the derivatization of carboxyl groups on peptides.Nearly 100% derivatization efficiency was achieved with the synthetic peptide RVYVHPI(RI-7).Furthermore, the peptide derivative was stable in a 0.1% TFA/water solution or a 0.1%(v/v) TFA/acetonitrile/water solution for at least one week.The effect of BAPI derivatization on the ionization of the peptide RI-7 was further investigated, and the detection sensitivity was improved >42-fold via matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS), thus outperforming the commercial piperazine derivatization approach.Moreover, the charge states of the peptide were largely increased via BAPI derivatization by electrospray ionization(ESI) MS.The results indicate the potential merits of BAPI derivatization for high sensitivity peptide analysis by MS. |
| 语种 | 英语 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/177455]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 中国科学院大连化学物理研究所 |
| 推荐引用方式 GB/T 7714 | Qiao Xiaoqiang,Zhou Yuan,Hou Chunyan,et al. 13aminopropyl3butylimidazoliumbromideforcarboxylgroupderivatizationpotentialapplicationsinhighsensitivitypeptideidentificationbymassspectrometry[J]. sciencechinalifesciences,2013,56(3):240. |
| APA | Qiao Xiaoqiang.,Zhou Yuan.,Hou Chunyan.,Zhang Xiaodan.,Yang Kaiguang.,...&Zhang Yukui.(2013).13aminopropyl3butylimidazoliumbromideforcarboxylgroupderivatizationpotentialapplicationsinhighsensitivitypeptideidentificationbymassspectrometry.sciencechinalifesciences,56(3),240. |
| MLA | Qiao Xiaoqiang,et al."13aminopropyl3butylimidazoliumbromideforcarboxylgroupderivatizationpotentialapplicationsinhighsensitivitypeptideidentificationbymassspectrometry".sciencechinalifesciences 56.3(2013):240. |
入库方式： OAI收割
来源：大连化学物理研究所
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