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  2. 合肥物质科学研究院
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  4. 中科院合肥智能机械研究所
NeuN-Specific Fluorescent Probe Revealing Neuronal Nuclei Protein and Nuclear Acids Association in Living Neurons under STED Nanoscopy

文献类型：期刊论文

作者Tian, Xiaohe1,3,4; Liu, Tianyan1; Fang, Bin4; Wang, Aidong2; Zhang, Mingzhu4; Hussain, Sajid1; Luo, Lei7; Zhang, Ruilong4; Zhang, Qiong4; Wu, Jieying4
刊名ACS APPLIED MATERIALS & INTERFACES
出版日期2018-09-26
卷号10期号:38页码:31959-31964
关键词fluorescent probe neuronal nuclei protein living neurons STED nanoscopy
ISSN号1944-8244
DOI10.1021/acsami.8b11102
通讯作者Zhang, Zhongping(zpzhang@iim.ac.cn) ; Tian, Yupeng(yptian@ahu.edu.cn)
英文摘要Neuronal nuclei (NeuN) protein is a key RNA-associated protein to guide the transcription of RNA and regulate mRNA splicing in neurons. However, the lack of effective labeling and tracking method has hindered the elucidation of the biological mechanism of NeuN operation in living neurons to understand correlated central nervous system disorders. Here, we report a molecular probe that can be inserted into a neighboring hydrophobic-hydrophilic region in NeuN, which upon binding becomes capable of emitting light in red region. The NeuN specificity enables the probe imaging neuronal cells in primary brain regions including hippocampus, cerebellum, midbrain, and cingulate gyrus. The probes' optical properties are such to enable stimulated emission depletion imaging showing for the first time the 3D structure of RNA tangling into NeuN in a living neuron with tens of nanometer resolution.
WOS关键词CELL ; MICROSCOPY
资助项目National Natural Science Foundation of China[21602003] ; National Natural Science Foundation of China[51432001] ; National Natural Science Foundation of China[51672002] ; National Natural Science Foundation of China[51772002] ; National Natural Science Foundation of China[81503009] ; National Natural Science Foundation of China[21501001] ; Anhui University Doctor Startup Fund[J01001962] ; Anhui Provincial Natural Science Foundation of China[1708085MC68] ; Anhui Provincial Returnees Innovation and Entrepreneurship key support program ; Open fund for Discipline Construction ; Institute of Physical Science and Information Technology, Anhui University
WOS研究方向Science & Technology - Other Topics ; Materials Science
语种英语
WOS记录号WOS:000446142100015
出版者AMER CHEMICAL SOC
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Anhui University Doctor Startup Fund ; Anhui University Doctor Startup Fund ; Anhui University Doctor Startup Fund ; Anhui University Doctor Startup Fund ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Returnees Innovation and Entrepreneurship key support program ; Anhui Provincial Returnees Innovation and Entrepreneurship key support program ; Anhui Provincial Returnees Innovation and Entrepreneurship key support program ; Anhui Provincial Returnees Innovation and Entrepreneurship key support program ; Open fund for Discipline Construction ; Open fund for Discipline Construction ; Open fund for Discipline Construction ; Open fund for Discipline Construction ; Institute of Physical Science and Information Technology, Anhui University ; Institute of Physical Science and Information Technology, Anhui University ; Institute of Physical Science and Information Technology, Anhui University ; Institute of Physical Science and Information Technology, Anhui University ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Anhui University Doctor Startup Fund ; Anhui University Doctor Startup Fund ; Anhui University Doctor Startup Fund ; Anhui University Doctor Startup Fund ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Returnees Innovation and Entrepreneurship key support program ; Anhui Provincial Returnees Innovation and Entrepreneurship key support program ; Anhui Provincial Returnees Innovation and Entrepreneurship key support program ; Anhui Provincial Returnees Innovation and Entrepreneurship key support program ; Open fund for Discipline Construction ; Open fund for Discipline Construction ; Open fund for Discipline Construction ; Open fund for Discipline Construction ; Institute of Physical Science and Information Technology, Anhui University ; Institute of Physical Science and Information Technology, Anhui University ; Institute of Physical Science and Information Technology, Anhui University ; Institute of Physical Science and Information Technology, Anhui University ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Anhui University Doctor Startup Fund ; Anhui University Doctor Startup Fund ; Anhui University Doctor Startup Fund ; Anhui University Doctor Startup Fund ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Returnees Innovation and Entrepreneurship key support program ; Anhui Provincial Returnees Innovation and Entrepreneurship key support program ; Anhui Provincial Returnees Innovation and Entrepreneurship key support program ; Anhui Provincial Returnees Innovation and Entrepreneurship key support program ; Open fund for Discipline Construction ; Open fund for Discipline Construction ; Open fund for Discipline Construction ; Open fund for Discipline Construction ; Institute of Physical Science and Information Technology, Anhui University ; Institute of Physical Science and Information Technology, Anhui University ; Institute of Physical Science and Information Technology, Anhui University ; Institute of Physical Science and Information Technology, Anhui University ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Anhui University Doctor Startup Fund ; Anhui University Doctor Startup Fund ; Anhui University Doctor Startup Fund ; Anhui University Doctor Startup Fund ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Returnees Innovation and Entrepreneurship key support program ; Anhui Provincial Returnees Innovation and Entrepreneurship key support program ; Anhui Provincial Returnees Innovation and Entrepreneurship key support program ; Anhui Provincial Returnees Innovation and Entrepreneurship key support program ; Open fund for Discipline Construction ; Open fund for Discipline Construction ; Open fund for Discipline Construction ; Open fund for Discipline Construction ; Institute of Physical Science and Information Technology, Anhui University ; Institute of Physical Science and Information Technology, Anhui University ; Institute of Physical Science and Information Technology, Anhui University ; Institute of Physical Science and Information Technology, Anhui University
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/39292]  
专题合肥物质科学研究院_中科院合肥智能机械研究所
通讯作者Zhang, Zhongping; Tian, Yupeng
作者单位1.Anhui Univ, Sch Life Sci, Hefei 230601, Anhui, Peoples R China
2.Huangshan Univ, Sch Chem & Chem Engn, Huangshan 245041, Peoples R China
3.Anhui Univ, Inst Phys Sci & Informat Technol, Hefei 230601, Anhui, Peoples R China
4.Anhui Univ, Dept Chem, Anhui Prov Key Lab Chem Inorgan Organ Hybrid Func, Hefei 230601, Anhui, Peoples R China
5.Nanjing Tech Univ, Key Lab Flexible Elect, Nanjing 211816, Jiangsu, Peoples R China
6.UCL, Dept Chem, London WC1H 0AJ, England
7.Southwest Univ, Coll Pharmaceut Sci, Chongqing 400716, Peoples R China
8.Nanjing Tech Univ, Inst Adv Mat, Jiangsu Natl Synergist Innovat Ctr Adv Mat SICAM, Nanjing 211816, Jiangsu, Peoples R China
9.Chinese Acad Sci, Inst Intelligent Machines, CAS Ctr Excellence Nanosci, Hefei 230000, Anhui, Peoples R China
推荐引用方式
GB/T 7714		 Tian, Xiaohe,Liu, Tianyan,Fang, Bin,et al. NeuN-Specific Fluorescent Probe Revealing Neuronal Nuclei Protein and Nuclear Acids Association in Living Neurons under STED Nanoscopy[J]. ACS APPLIED MATERIALS & INTERFACES,2018,10(38):31959-31964.
APA Tian, Xiaohe.,Liu, Tianyan.,Fang, Bin.,Wang, Aidong.,Zhang, Mingzhu.,...&Tian, Yupeng.(2018).NeuN-Specific Fluorescent Probe Revealing Neuronal Nuclei Protein and Nuclear Acids Association in Living Neurons under STED Nanoscopy.ACS APPLIED MATERIALS & INTERFACES,10(38),31959-31964.
MLA Tian, Xiaohe,et al."NeuN-Specific Fluorescent Probe Revealing Neuronal Nuclei Protein and Nuclear Acids Association in Living Neurons under STED Nanoscopy".ACS APPLIED MATERIALS & INTERFACES 10.38(2018):31959-31964.

入库方式： OAI收割

来源：合肥物质科学研究院

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